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Immunogen
A synthesized peptide derived from human CD34
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Antibody s target
Human CD34
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Antibody s full description
Rabbit Anti-Human CD34 Polyclonal
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Primary research fields
Immunology, Cell Type Markers, CD Markers, Stem Cells, Lineage Markers, Mesoderm, Stem Cells, Mesenchymal Stem Cells, Negative Markers, Stem Cells, Hematopoietic Progenitors, HP Surface Molecules , Stem Cells, Endothelial Progenitors
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Hematopoietic progenitor cell antigen CD34 Antibody, CD34 Antibody, Cluster designation 34 Antibody, Mucosialin Antibody, HPCA1 Antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total CD34
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1270 was sufficient for detection of CD34 in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane
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Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
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Scientific context
CD34, also known as gp105-120, is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. It is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation sites. It mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
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Bibliography
1. Sidney L.E., Branch M.J., Dunphy S.E., Dua H.S., Hopkinson A. (2014). Stem Cells 32(6): 1380–1389. 2. Simmons D.L., Satterthwaite A.B., Tenen D.G., Seed B. (1992). Journal of Immunology. 148 (1): 267–271. 3. Nielsen J.S., McNagny K.M. (2008). Journal of Cell Science 121 (Pt 22): 3683–3692. 4. more
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Released date
6/Sep/2016
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NCBI number
NP_001764.1
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Gene number
947
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Protein number
P28906
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Human HepG2 cell lysates showing detection of ~120kDa CD34 protein using Rabbit Anti-CD34 Polyclonal Antibody (SPC-1270). Lane 1: Human HepG2 cell lysate treated with the immunizing peptide. Lane 2: HumanHepG2 cell lysate. Primary Antibody: Rabbit Anti-CD34 Polyclonal Antibody (SPC-1270) at 1:1000. Predicted/Observed Size: ~120kDa. Rabbit Anti-CD34 Antibody used in Western blot (WB) on HepG2 cell lysates (SPC-1270)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Stem cell or hematopoietic marker. The most used affinity clone is QBEND10. We compare the affinity to this clone. This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
CD34
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Short name
Rabbit Anti- CD34 Polyclonal: FITC
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
FITC
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman CD34 molecule polyclonal: fluorecein
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Alternative technique
polyclonals, rabbit-anti, antibodies, fluorescine
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Alternative to gene target
CD34 molecule, CD34 and IDBG-106399 and ENSG00000174059 and 947, sulfate binding, Extracellular, Cd34 and IDBG-209317 and ENSMUSG00000016494 and 12490, CD34 and IDBG-638449 and ENSBTAG00000010562 and 281051
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