pVision-GFP-N Vector
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Catalog number9999-20
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PricePlease ask
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Size20 μg
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DescriptionAn eukaryotic expression vector encoding green fluorescent protein. pVisionGFP-N1 vector is a mammalian expression vector encoding green fluorescent protein, VisionGFP. pVisionGFP-N vector is designed to generate fusions to VisionGFP N-terminus for expression, localization and cellular dynamics studies or to express VisionGFP in eukaryotic (mammalian) cells. pVisionGFP-N vector carries synthetic version of the VisionGFP gene which codon usage is humanized, i.e. optimized for high expression in mammalian cells. pVisionGFP-N vector backbone contains immediate early promoter of cytomegalovirus (PCMV IE) for protein expression, SV40 origin for replication in mammalian cells, pUC origin of replication for propagation in E. coli, and f1 origin for single-stranded DNA production. SV40 early promoter provides neomycin resistance gene expression to select stably transfected eukaryotic cells using G418. Bacterial promoter (P) provides kanamycin resistance gene expression in E. coli. To increase VisionGFP mRNA translation efficiency, Kozak consensus translation initiation site is generated upstream of VisionGFP coding sequence. Multiple cloning site (MCS) is located between PCMV IE and VisionGFP coding sequence.
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Product Highlights• Amount - 20 µg • Application: N-terminal mammalian expression vector encoding humanized TagGFP and allowing TagGFP expression and generation of fusions to the TagGFP N-terminus. Generation of Vision GFP-tagged fusions A localization signal or a gene of interest should be cloned into MCS of the vector. It will be expressed as a fusion to VisionGFP N-terminus when inserted in the same reading frame as VisionGFP and no in-frame stop codons are present. VisionGFP-tagged fusions retain fluorescent properties of the native protein allowing fusion localization in vivo. Unmodified pVisionGFP-N vector will express VisionGFP when transfected into eukaryotic (mammalian) cells. Expression in Mammalian Cells pVisionGFP-N can be transfected into mammalian cells by any known transfection method. CMV promoter provides strong, constitutive expression of VisionGFP or VisionGFP-tagged fusions in many cell types. If required, stable transformants can be selected using G418. Propagation in E. coli • Suitable host strains: DH5alpha, HB101, and other general purpose strains. Single-stranded DNA production requires a host containing an F plasmid such as JM109 or XL1-Blue. • Selectable marker: plasmid confers resistance to kanamycin (30 μg/ml) to E. coli hosts. • E. coli replication origin: pUC • Copy number: ~500 • Plasmid incompatibility group: pMB1/ColE1 Generation of Vision GFP-tagged fusions A localization signal or a gene of interest should be cloned into MCS of the vector. It will be expressed as a fusion to VisionGFP N-terminus when inserted in the same reading frame as VisionGFP and no in-frame stop codons are present. VisionGFP-tagged fusions retain fluorescent properties of the native protein allowing fusion localization in vivo. Unmodified pVisionGFP-N vector will express VisionGFP when transfected into eukaryotic (mammalian) cells. Expression in Mammalian Cells pVisionGFP-N can be transfected into mammalian cells by any known transfection method. CMV promoter provides strong, constitutive expression of VisionGFP or VisionGFP-tagged fusions in many cell types. If required, stable transformants can be selected using G418. Propagation in E. coli -Suitable host strains: DH5alpha, HB101, and other general purpose strains. Single-stranded DNA production requires a host containing an F plasmid such as JM109 or XL1-Blue. -Selectable marker: plasmid confers resistance to kanamycin (30 μg/ml) to E. coli hosts. - E. coli replication origin: pUC -Copy number: ~500 -Plasmid incompatibility group: pMB1/ColE1
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Storage Temp-20°C
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Shippinggel pack
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Shelf Life12 months
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NotesFor research use only. Not to be used for human or animal treatment or consumption.
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Gene target
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Gene symbolGH1, POLM, NRAS, SDC3, PIGN, WASL, MAP6, HLA-N, FERD3L, SHC3
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Short namepVision-GFP-N Vector
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TechniqueVectors
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Labelgfp
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Alternative namepVision-Green Fluorescent-N integrating Desoxyribonucleic acid sequence
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Gene info
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Identity
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Gene
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Long gene namegrowth hormone 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Growth hormone family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameDNA polymerase mu
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Synonyms gene name
- polymerase (DNA directed), mu
- polymerase (DNA) mu
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-02-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- DNA polymerases
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameNRAS proto-oncogene, GTPase
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Synonyms gene name
- neuroblastoma RAS viral (v-ras) oncogene homolog
- neuroblastoma RAS viral oncogene homolog
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RAS type GTPase family
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namesyndecan 3
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Synonyms gene name
- syndecan 3 (N-syndecan)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1994-05-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Syndecans
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namephosphatidylinositol glycan anchor biosynthesis class N
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Synonyms gene name
- phosphatidylinositol glycan, class N
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-05-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Phosphatidylinositol glycan anchor biosynthesis
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameWASP like actin nucleation promoting factor
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Synonyms gene name
- Wiskott-Aldrich syndrome-like
- Wiskott-Aldrich syndrome like
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-10-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Wiskott-Aldrich Syndrome protein family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicrotubule associated protein 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-02-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, N (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-10-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameFer3 like bHLH transcription factor
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Synonyms gene name
- Fer3-like (Drosophila)
- Fer3-like bHLH transcription factor
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-12-09
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Entrez gene record
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Pubmed identfication
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Classification
- Basic helix-loop-helix proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSHC adaptor protein 3
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Synonyms gene name
- src homology 2 domain containing transforming protein C3
- SHC (Src homology 2 domain containing) transforming protein 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-11-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SH2 domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
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Tree numbers
- E05.393.350.800
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data