Goat anti Human alpha-1 antitrypsin
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Catalog numberGAHu/Atr
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PricePlease ask
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Size1 mL
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CategoryPrimary Antibodies
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Long descriptionThe reactivity of the antiserum is restricted to alpha-1antitrypsin. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified alpha-1antitrypsin. No reaction is obtained with any other plasma protein component or serum. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of alpha-1antitrypsin in human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive non-precipitating antibody-binding techniques if proper controls are included.
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Antibody come fromAlpha-1 antitrypsin is a serine protease inhibitor. It has a molecular weight of 54,000, its mean concentration in normal serum is 2.5 mg/ml. The highly purified immunogen is isolated from pooled normal plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other descriptionDelipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
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ClonePolyclonal
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Antigen antibody binding interactionGoat anti Human alpha-1 antitrypsin Antibody
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Antibody is raised inGoat
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Antibody s reacts withHuman
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Antibody s reacts with these speciesThe antiSerum does not cross react with any other component of Human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion a reaction has been found with Rhesus Monkey, Cynomolgus, Baboon and Chimpanzee; no reaction with Mouse.
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Antibody s specificityPrecipitating polyclonal Goat antiSerum to Human alpha-1 antitrypsin
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Research interestCardiovascular
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ApplicationImmunoprecipitation
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Antibody s suited forImmunoprecipitation. The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Precipitin titre 1:32 when tested against in agar-block immunodiffusion titration against an appropriate reference.
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StorageLyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
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Relevant referencesno information yet
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Protein numberP01009
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThe Goat anti alpha-1 antitrypsin is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Latin nameCapra aegagrus hircus
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Gene target
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Gene symbolSERPINA1, SERPINA2, SERPINA7
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Short nameGoat anti alpha-1 antitrypsin
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Techniqueanti human, anti, Goat, antibody to, anti human antibodies, goats
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotypenot specified
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Labelunlabeled
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SpeciesHuman, Goats, Humans
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Alternative namecaprine antibody to H. sapiens a-1 antitrypsin
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Alternative techniqueantibodies, caprine
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Gene info
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Identity
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Gene
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Long gene nameserpin family A member 1
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Synonyms gene
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Synonyms gene name
- serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
- serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Serpin peptidase inhibitors
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameserpin family A member 2 (gene/pseudogene)
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Synonyms gene
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Synonyms gene name
- serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 2
- serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 2, pseudogene
- serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 2 (gene/pseudogene)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Serpin peptidase inhibitors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameserpin family A member 7
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Synonyms gene
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Synonyms gene name
- serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
- serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Serpin peptidase inhibitors
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data