HSP70/HSC70, Heat shock protein 70/Heat shock cognate protein 70 (serum)

• Catalog number
  AS05 083
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Lyophilized
• Immunogen
  KLH-conjugated synthetic peptide conserved across all known sequences of HSP70 P08107 and HSC70 proteins P11142
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution add 100 µl of sterile water
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  Western blot (WB), immunoprecipitation (IP)
• Connected products
  Secondary antibodies
• Recommended dilutions for use
  1: 1000 with standard ECL (WB), 1: 10 000 with ECL Advance (GE Healthcare), 1: 1000 (IP)
• Molecular weight expected аpparent
  70 kDa
• Verified reactivity
  fish, mammals, fungi: Antrodia infirma, A. sinuosa, A. xantha, Catostomus commersonii, Gloeophyllum protractum, G. sepiarium, G. carbonarium, Oligoporus sericiomollis, Phlebia cornea, Junghunia luteoalba
• Possible reactivity
  vertebrates including bovine, hen, mouse, rat; insects including Drosophila melanogaster
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  This antibody is recognizing both, the inducible and the constitutive Hsp70.
• References
  MacLellan et al. (2015). Chaperone roles for TMAO and HSP70 during hyposmotic stress in the spiny dogfish shark (Squalus acanthias). J Comp Physiol B. 2015 Jun 7. Bessemer et al. (2014). Cardiorespiratory toxicity of environmentally relevant zinc oxide nanoparticles in the freshwater fish Catostomus commersonii. Nanotoxicology. 2014 Nov 27:1-10.Gorovits et al. (2013). Recruitment of the host plant heat shock protein 70 by tomato yellow leaf curl virus coat protein is required for virus infection. PLoS Once, July 23;8(7).
• Scientific context
  Heat shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stress or insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70) is a highly conserved protein and a member of the family of molecular chaperones. Alternative names: HSP70.1, HSP70-1/HSP70-2, Heat shock 70 kDa protein 8
• Notes
  for detection of plant and algal cytoplasmic hsp70 we recommend following product: AS08 371
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Description
  Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
• Group
  Sera
• About
  Serum should be stored at +5°C

• Gene target
  HSP70/HSC70   Heat   shock   protein   70/Heat   shock   cognate   protein  
• Gene symbol
  HSPA1B, HSPA14, HSPA1A, HSPA1L, HSPA8
• Short name
  HSP70/HSC70, Heat shock protein 70/Heat shock cognate protein 70 (serum)
• Technique
  serum
• Alternative name
  HSP70/HSC70, Heat shock protein 70/Heat shock cognate protein 70 (blood serum)
• Alternative technique
  sera
• Tissue
  serum

Gene info

• Identity
  HGNC:5233
• Gene
  HSPA1B
• Long gene name
  heat shock protein family A (Hsp70) member 1B
• Synonyms gene name
  • heat shock 70kD protein 1B
  • heat shock 70kDa protein 1B
• Synonyms
  • HSP70-2
• Locus
  6p21.33
• Discovery year
  1995-08-11
• Entrez gene record
  3304
• Pubmed identfication
  • 1700760
• Classification
  • TIM23 complex
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000031202

Gene info

• Identity
  HGNC:29526
• Gene
  HSPA14
• Long gene name
  heat shock protein family A (Hsp70) member 14
• Synonyms gene name
  • heat shock 70kDa protein 14
• Synonyms
  • HSP70-4
  • HSP70L1
• GenBank acession
  • AF112210
• Locus
  10p13
• Discovery year
  2004-04-20
• Entrez gene record
  51182
• Pubmed identfication
  • 21208456
• RefSeq identity
  • NM_016299
• Classification
  • Heat shock 70kDa proteins

Gene info

• Identity
  HGNC:5232
• Gene
  HSPA1A
• Long gene name
  heat shock protein family A (Hsp70) member 1A
• Synonyms gene
  • HSPA1
• Synonyms gene name
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
• Synonyms
  • HSP70-1
• GenBank acession
  • BC002453
• Locus
  6p21.33
• Discovery year
  2001-06-22
• Entrez gene record
  3303
• Classification
  • TIM23 complex
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000031201

Gene info

• Identity
  HGNC:5234
• Gene
  HSPA1L
• Long gene name
  heat shock protein family A (Hsp70) member 1 like
• Synonyms gene name
  • heat shock 70kD protein-like 1
  • heat shock 70kDa protein 1-like
• Synonyms
  • HSP70-HOM
  • hum70t
• GenBank acession
  • D85730
• Locus
  6p21.33
• Discovery year
  1989-05-12
• Entrez gene record
  3305
• Pubmed identfication
  • 9685725
  • 9349405
• Classification
  • TIM23 complex
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000031208

Gene info

• Identity
  HGNC:5241
• Gene
  HSPA8
• Long gene name
  heat shock protein family A (Hsp70) member 8
• Synonyms gene
  • HSPA10
• Synonyms gene name
  • heat shock 70kD protein 8
  • heat shock 70kDa protein 8
• Synonyms
  • HSC71
  • HSC70
  • HSP73
• GenBank acession
  • Y00371
• Locus
  11q24.1
• Discovery year
  1995-05-08
• Entrez gene record
  3312
• Pubmed identfication
  • 8530083
  • 3037489
• Classification
  • Spliceosomal P complex
  • Heat shock 70kDa proteins
  • Spliceosomal C complex
• VEGA ID
  OTTHUMG00000166030

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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