Cytochrome P450 3A4 (CYP3A4) Activity Assay Kit (Fluorometric)
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Catalog numberK701-200
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PricePlease ask
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Size200 assays
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DescriptionHighly Sensitive; Includes human CYP3A4 - NADPH Reductase as positive control.
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Summary• Detection method - Fluorescence (Ex/Em 535/587 nm) • Species reactivity - Eukaryotes • Applications - • Rapid assessment of native/recombinant CYP3A4 activity in lysates or microsomal fractions prepared from tissues, cells. • Screening of drugs and novel ligands for interaction with native/recombinant CYP3A4.
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Detection MethodFluorescence (Ex/Em 535/587 nm)
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Species ReactivityEukaryotes
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Applications• Rapid assessment of native/recombinant CYP3A4 activity in lysates or microsomal fractions prepared from tissues or cells. • Screening of drugs and novel ligands for interaction with native/recombinant CYP3A4.
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Sample Type• Human liver microsomes and liver S9 fractions • Lysates of tissues and cultured cells, primary hepatocytes • Heterologously expressed recombinant CYP3A4 preparations
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Features Benefits• Simple, convenient, highly sensitive • Fluorescent assay enables determination of CYP3A4, CYP3A5 activity in a variety of biological samples • The substrate shows low background and a high signal-to-noise ratio • Kit includes CYP3A inhibitor ketoconazole and a stable, recombinant human CYP3A4 co-expressed with NADPH Reductase as a positive control
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundCytochrome P450 3A4 (CYP3A4, EC 1.14.13.157) is a member of the cytochrome P450 monooxidase (CYP) family of microsomal xenobiotic metabolism enzymes. CYPs are membrane-bound hemeproteins responsible for Phase I biotransformation reactions, in which lipophilic drugs and other xenobiotic compounds are transformed to more hydrophilic products to facilitate excretion from the body. CYP3A4 is expressed in high levels in the liver and intestines, where it catalyzes oxidation of an extraordinarily wide variety of structurally distinct ligands. More than half of all small molecule drugs commonly used by humans are metabolized by CYP3A4. Inhibition of CYP3A4-mediated metabolism is a common cause of adverse drug/drug and drug/food interactions and toxicity. In addition, for drugs whose pharmacological activity requires metabolism from a pro-drug form, CYP3A4 inhibition can lead to decreased drug efficacy. BioVision’s CYP3A4 Activity Assay Kit enables rapid measurement of native or recombinant CYP3A4 activity in biological samples such as liver microsomes. The assay utilizes a non-fluorescent CYP3A4 substrate that is converted into a highly fluorescent metabolite detected in the visible range (Ex/Em = 535/587 nm), ensuring a high signal-to-background ratio with little interference by autofluorescence. CYP3A4 specific activity is calculated by running parallel reactions in the presence and absence of the potent inhibitor Ketoconazole and subtracting any residual activity detected with the inhibitor present. The kit contains a complete set of reagents sufficient for performing 100 sets of paired reactions (in the presence and absence of inhibitor).
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TestBiovision supplies other types of Assays as 1.
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Gene target
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Gene symbolCYP3A4, CYP3A7, CYP24A1, CYP1A1, CYP51A1, CYP2D6
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Short nameCytochrome P450 3A4 (CYP3A4) Activity Assay Kit (Fluorometric)
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TechniqueAssay, assays
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HostAssay
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Alternative nameCytochrome P450 3A4 (CYP3A4) Activity test reagent (fluorescence)
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Alternative techniquearrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 3 subfamily A member 4
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Synonyms gene
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Synonyms gene name
- cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4
- cytochrome P450, family 3, subfamily A, polypeptide 4
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GenBank acession
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Locus
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Discovery year1990-02-24
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Entrez gene record
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Pubmed identfication
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Classification
- Cytochrome P450 family 3
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 3 subfamily A member 7
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Synonyms gene name
- cytochrome P450, subfamily IIIA, polypeptide 7
- cytochrome P450, family 3, subfamily A, polypeptide 7
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-09-14
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Entrez gene record
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Pubmed identfication
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Classification
- Cytochrome P450 family 3
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 24 subfamily A member 1
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Synonyms gene
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Synonyms gene name
- cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
- cytochrome P450, family 24, subfamily A, polypeptide 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-09-09
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Entrez gene record
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Classification
- Cytochrome P450 family 24
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 1 subfamily A member 1
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Synonyms gene
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Synonyms gene name
- cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1
- cytochrome P450, family 1, subfamily A, polypeptide 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cytochrome P450 family 1
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 51 subfamily A member 1
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Synonyms gene
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Synonyms gene name
- cytochrome P450, 51 (lanosterol 14-alpha-demethylase)
- cytochrome P450, family 51, subfamily A, polypeptide 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-10-26
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Entrez gene record
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Pubmed identfication
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Classification
- Cytochrome P450 family 51
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 2 subfamily D member 6
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Synonyms gene
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Synonyms gene name
- cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolizing), polypeptide 6
- cytochrome P450, family 2, subfamily D, polypeptide 7 pseudogene 2
- cytochrome P450, subfamily II (debrisoquine, sparteine, etc., -metabolising), polypeptide 7 pseudogene 2
- cytochrome P450, family 2, subfamily D, polypeptide 8 pseudogene 2
- cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolising), polypeptide 8 pseudogene 2
- cytochrome P450, family 2, subfamily D, polypeptide 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-04-07
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Entrez gene record
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Pubmed identfication
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Classification
- Cytochrome P450 family 2
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VEGA ID
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Locus Specific Databases
