FlowTACS Kit

Keep at

Mixed
Reagent list
Assay performance

TACS for in situ detection of apoptosis in a TUNEL test with frozen, paraffin embedded tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA with enzymes and permeabilization reagents, labeling and detection reagents, stop buffers, counterstain and TACS-Nuclease for in situ labeling.
More advice

Streptavidin HRP and and TACS Nucleases for positive controls are supplied for immunohistochemistry with HRP an DAB with incorporation of bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA for apoptosis detection.
Other trevi products

Gene target

FlowTACS Kit
Short name

FlowTACS Kit
Alternative name

FlowTACS reagent
Alternative technique

kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Name

Flow Cytometry
Concept

Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data