AMACR Antibody

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• Catalog number
  V2506IHC-7ML
• Price:

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• Size
  7 ml

# #

• Category
  Antibody
• Concentration
  Prediluted in 1X PBS, 0.05% sodium azide; *For IHC use only*
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  AMACR
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens). Other species not tested. ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  IHC
• Recommended dilutions
  Prediluted format : incubate for 30 min at RT (2)
• Notes
  Optimal dilution of the AMACR antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Intented use
  This AMACR antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q9UHK6
• Purity
  Purified by protein A Affinity Chromatography
• Description
  This antibody recognizes a protein of 54kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.
• Immunogen
  A synthetic peptide from the human protein was used as the immunogen for the AMACR antibody.
• Storage
  Store the AMACR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
• Localization
  Cytoplasmic
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

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• Gene target
  AMACR  
• Gene symbol
  C1QTNF3-AMACR, AMACR
• Short name
  Anti-AMACR
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to AMACR
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:49198
• Gene
  C1QTNF3-AMACR
• Long gene name
  C1QTNF3-AMACR readthrough (NMD candidate)
• Locus
  5p13.2
• Discovery year
  2013-09-30
• Entrez gene record
  100534612
• VEGA ID
  OTTHUMG00000186337

Gene info

• Identity
  HGNC:451
• Gene
  AMACR
• Long gene name
  alpha-methylacyl-CoA racemase
• Synonyms
  • RACE
  • P504S
• GenBank acession
  • AF047020
• Locus
  5p13.2
• Discovery year
  1999-10-19
• Entrez gene record
  23600
• Pubmed identfication
  • 9307041
• RefSeq identity
  • NM_014324
• VEGA ID
  OTTHUMG00000090734

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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