Human Smad3/MADH3(Mothers Against Decapentaplegic Homolog 3) EIA Kit

• Catalog number
  E-EL-H1324-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Human
• Target Synonym
  NA
• Test principle
  This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human Smad3/MADH3. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Smad3/MADH3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Smad3/MADH3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Smad3/MADH3. You can calculate the concentration of Human Smad3/MADH3 in the samples by comparing the OD of the samples to the standard curve.
• Sensitivity
  0.38ng/mL
• Detection Range
  0.63~40ng/mL
• Target Name
  Smad3/MADH3
• UNIProt ID
  P84022
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Human Smad3/MADH3(Mothers Against Decapentaplegic Homolog 3) EIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Description
  This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
• Properties
  MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

• Gene target
  Smad3/MADH3   Mothers   Against   Decapentaplegic   Homolog   Kit  
• Gene symbol
  SMAD3
• Short name
  Smad3/MADH3(Mothers Against Decapentaplegic Homolog 3) EIA Kit
• Technique
  EIA, eia
• Species
  Human, Humans
• Alternative name
  H. sapiens SMAD family member 3/MADH3(Mothers Against Decapentaplegic Homolog 3) EIA reagent
• Alternative technique
  kits, elisas
• Alternative to gene target
  SMAD family member 3, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, transforming growth factor beta receptor, nuclei, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125

Gene info

• Identity
  HGNC:6769
• Gene
  SMAD3
• Long gene name
  SMAD family member 3
• Synonyms gene
  • MADH3
• Synonyms gene name
  • MAD, mothers against decapentaplegic homolog 3 (Drosophila)
  • SMAD, mothers against DPP homolog 3 (Drosophila)
• Synonyms
  • JV15-2
  • HsT17436
• GenBank acession
  • BC050743
• Locus
  15q22.33
• Discovery year
  1996-11-15
• Entrez gene record
  4088
• Pubmed identfication
  • 8774881
  • 8673135
• RefSeq identity
  • NM_005902
• Classification
  • SMAD family
• VEGA ID
  OTTHUMG00000133230
• Locus Specific Databases
  • The Globin Gene Server

MeSH Data

• Name
  Immunoenzyme Techniques
• Concept
  Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
• Tree numbers
  • E05.478.566.350
  • E05.478.583.400
  • E05.601.470.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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