Detection limits
78.12-5000 pg/mL
Sensitivity limit
31,5 pg/mL
Samples to be analyzed
Tissue homogenates, cell lysates and other biological fluids.
Protein number
Q9BWD1
Gene number
39
Gene name
Acetyl Coenzyme A Acetyltransferase 2
Alternate gene name
ACTL; Acetoacetyl Coenzyme A Thiolase; Acetyl-CoA transferase-like protein; Cytosolic acetoacetyl-CoA thiolase
ELISA's specificity
This assay has high sensitivity and excellent specificity for detection of Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). No significant cross-reactivity or interference between Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) and analogues was observed.
Assay principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Acetyltransferase 2 (ACAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Precision of the test
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
Research main area
Enzyme & Kinase;
Description
N terminal acetylation or CH3CO as epigenetic regulation of Coenzyme A Acetyltransferase 2 (ACAT2) ELISA Kit, 96 by NATs.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals.
A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
