FAK Antibody

• Catalog number
  3399-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  FAK
• Target Alternative Name
  FAK , FAK1 , FAK-1 , focal adhesion kinase 1 , PTK2, protein tyrosine kinase 2 , FADK1 , pp125FAK
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Recombinant human FAK
• Accession
  NP_005598
• Gene Id
  5747
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot, Immunoprecipitation, Immunofluorescence
• Application And Usages
  Western blotting (1-2 µg/ml), immunoprecipitation (5-20 µg/ml), and immunocytochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 125 kDa FAK of human, mouse, and rat origins.
• Description
  Focal adhesion kinase was identified as a 125 kDa substrate for the intrinsic protein tyrosine kinase activity of Src encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence sμggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only those basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds and is activated and localized to the focal adhesions of spreading keratinocytes in culture. Thus, it has been postulated that FAK may have an important in vivo role in the reepithelialization of human wounds. FAK protein tyrosine kinase activity has also been shown to increase in cells stimulated to grow by use of mitogenic neuropeptides or neurotransmitters acting throμgh G protein coupled receptors.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  FAK  
• Gene symbol
  PTK2
• Short name
  FAK Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  FAK (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:9611
• Gene
  PTK2
• Long gene name
  protein tyrosine kinase 2
• Synonyms gene name
  • PTK2 protein tyrosine kinase 2
• Synonyms
  • FAK
  • FADK
  • FAK1
  • PPP1R71
• Synonyms name
  • protein phosphatase 1, regulatory subunit 71
• GenBank acession
  • L13616
• Locus
  8q24.3
• Discovery year
  1992-10-02
• Entrez gene record
  5747
• Pubmed identfication
  • 8422239
• RefSeq identity
  • NM_005607
• Classification
  • Protein phosphatase 1 regulatory subunits
  • FERM domain containing
  • PTK2 family tyrosine kinases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000067438

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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