Hamster Anti-Mouse CD30-LE/AF
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Catalog numberGEN673318
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PricePlease ask
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Size500ug
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Also known asCD30
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Other namesCD30 antigen; Tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily member 8; CD30 antigen; Ki-1 antigen; CD30L receptor; OTTHUMP00000001783; OTTHUMP00000221481; cytokine receptor CD30; lymphocyte activation antigen CD30; tumor necrosis factor receptor superfamily, member 8; CD30L receptor; Ki-1 antigen; Lymphocyte activation antigen CD30
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CategorySecondary Antibodies
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SubcategoryMnoclonal antibodies
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Gene nameTNFRSF8; TNFRSF8; CD30; Ki-1; D1S166E; CD30; D1S166E
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Gene name synonimsTNFRSF8; TNFRSF8; CD30; Ki-1; D1S166E; CD30; D1S166E
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Other gene namesTNFRSF8; TNFRSF8; CD30; Ki-1; D1S166E; CD30; D1S166E
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ClonalityMonoclonal
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Immunoglobulin isotypeArmenian Hamster IgG kappa
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Clone2SH12-5F (also known as mCD30.1)
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Host organismArmenian Hamster
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Species reactivityMouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivityMurine CD30, Mr 52 kDa (core peptide) CD30 is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily of proteins.^1,2 Murine CD30 is expressed predominantly in the thymus, and it is inducible in mouse splenocytes stimulated with pokeweed mitogen or concanavalin A.^1 In anti-CD3 epsilon -activated spleen cells, CD30 is expressed primarily on the surface of CD8^+ T cells with peak expression on days 4 and 5. Stimulation of CD30^+ CTL lines with plate-bound anti-CD30 directly signals IL-5 but not IFN- gamma production. ^1 While these studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 may play a pivotal role in the pattern of cytokine production by T cells, the precise roles of CD30 and its ligand (CD153) in T-cell development have not been clearly defined.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodN/A
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Form AppearanceLow Endotoxin, Azide - Free (LE/AF)
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ConcentrationN/A
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Storage and shippingThe purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below Store the antibody at -20 degrees Celsius.. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product the antibody should be stored at -20 degrees Celsius. immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
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Tested applicationsFlow Cytometry
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesArmenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolFUT3, HPS4, TNFRSF8, NPAS4
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Short nameAnti-Mouse CD30-LE/AF
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Techniqueanti-, Mouse, anti, antibody to, antibodies, mouses
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HostHamster
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SpeciesMouse, Hamsters, Mouses
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Alternative nameAnti-Hamster Anti-Mouse CD30-LE/AF
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene namefucosyltransferase 3 (Lewis blood group)
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Synonyms gene
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Synonyms gene name
- fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group included)
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Synonyms
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Synonyms name
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Locus
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Discovery year1993-04-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fucosyltransferases
- Blood group antigens
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameHPS4 biogenesis of lysosomal organelles complex 3 subunit 2
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Synonyms gene name
- Hermansky-Pudlak syndrome 4
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Synonyms
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Locus
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Discovery year2001-06-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Biogenesis of lysosomal organelles complex 3
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameTNF receptor superfamily member 8
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor receptor superfamily, member 8
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-01-10
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Entrez gene record
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Pubmed identfication
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Classification
- CD molecules
- Tumor necrosis factor receptor superfamily
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameneuronal PAS domain protein 4
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Basic helix-loop-helix proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data