pCR4-TOPO-PIK3CA Plasmid

Quantity:

Kit

Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.

Gene target

pCR4-TOPO-PIK3CA
Gene symbol

PIK3CA-DT, PIK3CA
Short name

pCR4-TOPO-PIK3CA Plasmid
Technique

plasmid, plasmids in 1
Alternative name

pCR4-TOPO-phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit a Plasmid
Alternative technique

plasmids
Alternative to gene target

phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha, CLOVE and CWS5 and MCAP and MCM and MCMTC and p110-alpha and PI3K, PIK3CA and IDBG-66003 and ENSG00000121879 and 5290, transferase activity, Plasma membranes, Pik3ca and IDBG-136530 and ENSMUSG00000027665 and 18706, PIK3CA and IDBG-636081 and ENSBTAG00000009232 and 282306

Quantity:

Identity

HGNC:8975
Gene

PIK3CA
Long gene name

phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Synonyms gene name
- phosphoinositide-3-kinase, catalytic, alpha polypeptide
- phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
Synonyms
- PI3K
Locus

3q26.32
Discovery year

1994-07-15
Entrez gene record

5290
Pubmed identfication
- 1322797
Classification
- Phosphatidylinositol 3-kinase subunits
VEGA ID

OTTHUMG00000157311
Locus Specific Databases
- LRG_310

Name

Polymerase Chain Reaction
Concept

Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Tree numbers
- E05.393.620.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data