Live/Dead Cell Viability Assay Kit (for Mammalian Cells)

• Catalog number
  K502-100
• Price
  Please ask
• Size
  100 assays

• Description
  Easy, non-radioactive assay kit to measure cell viability/proliferation, cytotoxicity and apoptosis by microscopy/FACS analysis.
• Summary
  • Detection method- Fluorescent microscopy or FACS analysis (Ex/Em 485/530 nm) to detect stained live cells; (Ex/Em 495/635) to detect stained dead cells • Species reactivity- Mammalian • Applications- Stained live and dead cells can easily be visualized by fluorescence microscopy or FACS analysis.
• Detection Method
  Detection method- Fluorescent microscopy or FACS analysis (Ex/Em 485/530 nm) to detect stained live cells; (Ex/Em 495/635) to detect stained dead cells
• Species Reactivity
  Mammalian
• Applications
  • Screening/studying/characterization of stimulators/inhibitors that affect cell viability.
• Sample Type
  ·    Adherent cells or suspension cells
• Features Benefits
  Easy to use Protocol Non-radioactive detection. Detect using either microscopy or FACS. Measure Cell Proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Quantification of number of live and dead cells is an indispensable tool in cell biology research. BioVision’s Live/Dead Cell Viability Assay Kit, provides a two-color fluorescence method that is based on the simultaneous determination of live and dead cells using two different dyes. Live cell dye easily penetrates intact, live cells and intracellular esterase hydrolyzes the dye to produce a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm which can be measured at Ex/Em = 485/530 nm. Dead cell dye enters damaged cell membranes and undergoes a 40-fold enhancement of fluorescence upon binding to nucleic acid, thereby producing a bright red fluorescence (Ex/Em = 495/635 nm) in dead cells. This assay kit provides an easy-to-use, non-radioactive, histological and FACS-based method for measuring cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Live/Dead   Viability   Kit   for   Mammalian   Cells  
• Short name
  Live/Dead Viability Assay Kit (for Mammalian Cells)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Live/Dead cellular cytotoxity test reagent (to measure Mammalian Cells)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell, cells

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