Mouse anti p53 (a.a. 16-25)

#

• Catalog number
  X1157M
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• Size
  100 μg

# #

• Category
  Primary Antibodies
• Long description
  p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.
• Antibody come from
  Hybridoma produced by the fusion of splenocytes from immunized with full length Xenopus p53 protein and mouse myeloma cells.
• Other description
  Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
• Clone
  X77
• Antigen antibody binding interaction
  Mouse anti p53 (a.a. 16-25) Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human, Mouse, Xenopus
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Western Blot, ELISA, Immunoprecipitation
• Antibody s suited for
  Detects p53 protein by Western blot at 1 to 10 µg/ml. Detects a band a approximately 53 kDa in HCT116 cell lysate. Optimal concentration should be evaluated by serial dilutions.
• Storage
  -20ºC
• Relevant references
  1. Zeng, X., et al. UV but not gamma irradiation accelerates p53-induced apoptosis of teratocarcinoma cells by repressing MDM2 transcription. Cancer Res. 2000, 60, 6184-6188_x000B__x000B_2. Hussain, S.P., et al. p53 tumor suppressor gene: at the crossroads of molecular carcinogenesis, molecular epidemiology and human risk assessment. Ann. N.Y. Acad. Sci. 2000, 919, 79-85_x000B__x000B_3. Hellin, A.C., et al. Roles of Nuclear Factor-kappaB, p53 and p21/WAF1 in Daunomycin-induced cell cycle arrest and apoptosis. J. Pharmacol. Exp. Ther. 2000, 295, 870-878_x000B__x000B_4. Portefaix, J.M., et al. 'Critical residues of epitopes recognized by several anti-p53 monoclonal antibodies correspond to key residues of p53 involved in interactions with the mdm2 protein.' J. Immunol. Methods. 2000, 244(1-2), 17-28.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

#

• Gene target
  p53   16-25  
• Gene symbol
  SNORD116-25, ZNF211, SNORD114-25, SNORD115-25, ESB3, ERVW-25, IGKV3D-25, IGLV3-25, RNVU1-25
• Short name
  Mouse anti p53 (a a. 16-25)
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to p53 (a.a. 16-25)
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:33091
• Gene
  SNORD116-25
• Long gene name
  small nucleolar RNA, C/D box 116-25
• Synonyms
  • HBII-85-25
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033436
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003339
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:13003
• Gene
  ZNF211
• Long gene name
  zinc finger protein 211
• Synonyms
  • ZNF-25
  • CH2H2-25
• GenBank acession
  • U38904
• Locus
  19q13.4
• Discovery year
  1999-09-29
• Pubmed identfication
  • 7633419
  • 9096115
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000168012

Gene info

• Identity
  HGNC:33013
• Gene
  SNORD114-25
• Long gene name
  small nucleolar RNA, C/D box 114-25
• Synonyms
  • 14q(II-25)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767605
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003218.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33044
• Gene
  SNORD115-25
• Long gene name
  small nucleolar RNA, C/D box 115-25
• Synonyms
  • HBII-52-25
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033801
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003342.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:3464
• Gene
  ESB3
• Long gene name
  esterase B3
• Locus
  16
• Discovery year
  2001-06-22
• Pubmed identfication
  • 3481956

Gene info

• Identity
  HGNC:49313
• Gene
  ERVW-25
• Long gene name
  endogenous retrovirus group W member 25
• Synonyms gene name
  • endogenous retrovirus group W, member 25
• GenBank acession
  • HG421063
• Locus
  13q13.3
• Discovery year
  2013-10-21

Gene info

• Identity
  HGNC:5826
• Gene
  IGKV3D-25
• Long gene name
  immunoglobulin kappa variable 3D-25 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 3D-25
  • immunoglobulin kappa variable 3D-25 pseudogene
• GenBank acession
  • X71886
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28873
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151609

Gene info

• Identity
  HGNC:5908
• Gene
  IGLV3-25
• Long gene name
  immunoglobulin lambda variable 3-25
• GenBank acession
  • X97474
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28793
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151221

Gene info

• Identity
  HGNC:54428
• Gene
  RNVU1-25
• Long gene name
  RNA, variant U1 small nuclear 25
• Locus
  1q21.1
• Discovery year
  2019-07-26
• Classification
  • Variant U1 small nuclear RNAs

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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