Biotin-labeled Rabbit anti BSA, affinity purified

Test

A high affinity purification column was use to purify - Rabbit anti BSA, by Molecular Innovations by chromatographic size exclusion.
Description

1 of protein A or G purified by Molecular Innovations will give more specificity than crude serum. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. FITC labelling for flow cytometry of antibodies and peptides.
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
About

Rabbits are used for polyclonal antibody production by Molecular Innovations. Rabbit antibodies are very stable and can be stored for several days at room temperature. Molecular Innovations adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name

Oryctolagus cuniculus

Gene target

BSA
Short name

- Rabbit anti BSA,
Technique

affinity, purified, Rabbit, anti, antibody to, purifications
Host

Rabbit, Rabbits
Label

Biotin
Alternative name

biotinilated-labeled production species: rabbit antibody to cow serum albumin, protein enriched
Alternative technique

pure, rabbit-anti, antibodies

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data