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Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr219 and Tyr221 of human ERK2 (AA 216-222)
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Antibody s target
Human ERK5 (pThr219 + pTyr221)
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Antibody s full description
Rabbit Anti-Human ERK5 (pThr219 + pTyr221) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Big MAP kinase 1 Antibody, BMK 1 kinase Antibody, ERK5 Antibody, BMK1 Antibody, EC 2.7.11.24 Antibody, ERK4 Antibody, ERK5 Antibody, Extracellular signal regulated kinase 5 Antibody, MAP kinase 7 Antibody, MAPK7 Antibody , Mitogen activated protein kinase 7 Antibody, MK07_HUMAN Antibody, OTTHUMP00000065906 Antibody, OTTHUMP00000065907 Antibody, PRKM7 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 88.637 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-977 was sufficient for detection of ERK5 (pThr219 + pTyr221) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus
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Tissue specificity
Highly expressed in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver
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Scientific context
ERK5 or MAPK7 is a protein-serine/threonine kinase. ERK5 is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). Upregulation of this gene has been found in breast, prostate, and liver cancer.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_002740.2
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Gene number
5598
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Protein number
Q13164
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
MAPK7, APC
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Short name
Rabbit Anti- ERK5 (pThr219 + pTyr221) Polyclonal: APC
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
APC
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman ERK5 (pThr219 + pTyr221) polyclonal: adenomatous polyposis coli
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
adenomatous polyposis coli, BTPS2 and DP2 and DP2.5 and DP3 and GS and PPP1R46, APC and IDBG-37007 and ENSG00000134982 and 324, microtubule plus-end binding, nuclei, Apc and IDBG-134573 and ENSMUSG00000005871 and 11789, BT.25770 and IDBG-644937 and ENSBTAG00000018852 and 533233
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