TPO Recombinant Protein

• Catalog number
  40-610
• Price
  Please ask
• Size
  0.01 mg

• About recombinant protein
  TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein.
• Other name of the recombinant
  ML, TPO, MGDF, MKCSF, MPLLG, THCYT1, Thrombopoietin, C-mpl ligand, ML
• Recombinant in
  E. coli
• His tag on the recombinant protein
  no His tag
• SEQUENCE
  SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL
• Activity
  The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be = 1.0 ng/mL, corresponding to a specific activity of = 1 x 106 units/mg.
• Keep at
  The lyophilized TPO recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted TPO is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing TPO recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  4507493
• Accession nr
  NP_000451.1
• Pureity
  Greater than 98% by SDS-PAGE gel and HPLC analyses.
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  7066
• Gene name
  thrombopoietin
• Source
  Homo sapiens
• Uniprot
  P40225
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  TPO   Protein  
• Gene symbol
  THPO
• Short name
  TPO Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  TPO Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:11795
• Gene
  THPO
• Long gene name
  thrombopoietin
• Synonyms gene
  • MGDF
• Synonyms
  • TPO
  • MPLLG
• Synonyms name
  • prepro-thrombopoietin
  • megakaryocyte stimulating factor
  • myeloproliferative leukemia virus oncogene ligand
  • megakaryocyte growth and development factor
  • MPL ligand
  • megakaryocyte colony-stimulating factor
  • c-mpl ligand
  • thrombopoietin nirs variant 1
• Locus
  3q27.1
• Discovery year
  1994-11-04
• Entrez gene record
  7066
• Pubmed identfication
  • 8202154
• RefSeq identity
  • NM_000460
• Classification
  • Receptor ligands
• VEGA ID
  OTTHUMG00000156745
• Locus Specific Databases
  • LRG_580

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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