PCAF Antibody (CT)
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Catalog number6763-100
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Price:
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Size100 μl
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Antibody TargetPCAF
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Target Alternative NameKAT2B; PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; Lysine acetyltransferase 2B; P300/CBP-associated factor.
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Antibody TypePolyclonal
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Species ReactivitiesHuman. Predicted cross reactivity with mouse samples.
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Immunogen SequenceThis PCAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 802-832 amino acids from the C-terminal region of human PCAF.
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AccessionQ92831
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Gene Id8850
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AppearanceColorless liquid
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FormLiquid
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Formulation100 µl of antibody in PBS with 0.09% (W/V) sodium azide
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PurificationThis antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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ApplicationWestern blot.
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Positive ControlWestern blot: U251 cell lysate
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Application And UsagesWestern blot: ~1:1000.
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DescriptionThe acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. P300/CBP-associated factor (PCAF) is a transcriptional coactivator that works both as a histone lysine acetyltransferase, through its HAT domain, and as an acetyl-lysine reader through its conserved bromodomain located directly C-terminal to the HAT domain. The PCAF bromodomain binds acetylated histone H3 and H4 as well as non-histone targets. Bromodomain binding is dictated by the position of the acetylated lysine as well as interactions with specific residues flanking the acetyl-lysine. PCAF also specifically binds the HIV viral protein Tat on acetylated K50 to regulate its transactivating activity and help induce chromatin remodeling of proviral genes, thereby promoting transcription of viral proteins.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolKAT2A, KAT2B
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Short namePCAF Antibody (CT)
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative namePCAF (antibody to-) (CT)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namelysine acetyltransferase 2A
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Synonyms gene
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Synonyms gene name
- GCN5 general control of amino-acid synthesis 5-like 2 (yeast)
- K(lysine) acetyltransferase 2A
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-12-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- GCN5 related N-acetyltransferases
- ATAC complex
- SAGA complex
- Lysine acetyltransferases
- Bromodomain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelysine acetyltransferase 2B
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Synonyms gene
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Synonyms gene name
- p300/CBP-associated factor
- K(lysine) acetyltransferase 2B
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-02-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- Bromodomain containing
- GCN5 related N-acetyltransferases
- ATAC complex
- SAGA complex
- Lysine acetyltransferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data