-
Products_type
Secondary Antibody
-
Reactivity
Mouse.Cross Reactivity: Based on IEP, no reactivity is observed to:non-immunoglobulin mouse serum immunoglobulinsHuman IgG or serum proteins
-
Test
A high affinity purification column was use to purify Goat anti-Mouse IgG (H L), F(ab)'2 - Pure, FITC Conjugate, min x w/ IgG serum proteins[IgG (H L), F(ab)'2 ] by MyBioSource by chromatographic size exclusion. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure Goat anti-Mouse IgG (H L), F(ab)'2 - Pure, FITC Conjugate, min x w/ IgG serum proteins[IgG (H L), F(ab)'2 ] supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
-
Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Conjugation
Anti-FITC Antibody
-
Latin name
Capra aegagrus hircus, Mus musculus
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Serum should be stored at +5°C
-
Group
Sera
-
Gene target
-
Short name
Goat anti-Mouse IgG (H L), F(ab)'2 - Pure, FITC Conjugate, min x w/ IgG serum proteins[IgG (H L), F(ab)'2 ]
-
Technique
affinity, anti-, Mouse, serum, anti, FITC, Goat, Pure, IgG, antibody to, antibodies, Fluorescein, goats, IgGs, mouses, pure products
-
Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
-
Isotype
IgG, Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common. Heavy & Light chains, IgG
-
Label
FITC
-
Species
Human, Goats, Humans, Mouses
-
Alternative name
caprine Antibody toMouse Immunoglobulin G (heavy + light chain), F(Antibody)'2 Fragment - protein Pure, fluorecein Conjugate, min x w/H. sapiens Immunoglobulin G and blood serum proteins[Immunoglobulin G (heavy + light chain), F(Antibody)'2 Fragment]
-
Alternative technique
sera, murine, antibodies, purified, caprine, fluorescine, immunoglobulins
-
Tissue
serum
-
