This antibody has been succesfully tested for use in following methods:
Western blot, IF, ELISA, ChIP, Dot blot.
Target of this antibody:
Formulation of this antibody:
In PBS with 0.05% sodium azide and 0.05% ProClin 300.
Alternative names of antibody target:
WB: 1:1000, ELISA: 1:1000, Dot Blot: 1:50,000, IF: 1:500, ChIP: 2 µg/ChIP.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Clonality of antibody:
KLH-conjugated synthetic peptide of Histone H3 containing dimethylated lysine 27.
How to handle this antibody:
The antibody solution should be gently mixed before use.
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Levels of H3K27 dimethylation are higher in silent genes than in active genes suggesting that this histone modification is associated with transcriptional repression.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
anti-H3K27me2 polyclonal antibody
Polyclonal, Antibody, anti
antibody to-H3K27me2 polyclonal (Antibody to)