Anti-Reverse Triiodothyronine (rT3)-Rabbit polyclonal Antibody
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Catalog number
PAC022Ge01
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Price
Please ask
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Size
10ug
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Organism Species
Pan-species (General)
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Source
Polyclonal antibody preparation
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Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
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Item Name
Reverse Triiodothyronine
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Immunogen
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Image number
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Species reactivity
General
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Sequence of immunogen
Small Molecule, rT3 conjugated to OVA
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Aplication
WB; IHC; ICC; IP.
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Clonality
Rabbit polyclonal
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Concentration
500ug/ml
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Alternative Names
RT3; r-T3; Reverse T3;(2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine
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Applicable Secondary Antibody
SAA544Rb59, SAA544Rb58, SAA544Rb57, SAA544Rb18, SAA544Rb19
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Delivery condition
4℃ with ice bags
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Storage instructions
Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by Cloud Clone Corp. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cloud Clone Corp adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Goup
reverse transcription
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French translation
anticorps
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Gene target
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Short name
Anti-Reverse Triiodothyronine (rT3)-Rabbit polyclonal Antibody
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Technique
Polyclonal, Antibody, reverse, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Alternative name
Antibody toReverse Triiodothyronine (rT3)-production species: rabbit polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies, transctription, rabbit-anti
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MeSH Data
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Name
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Concept
Scope note:
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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