Total Collagen Assay Kit (Colorimetric)

• Catalog number
  K218-100
• Price
  Please ask
• Size
  100 assays

• Description
  A simple, sensitive & easy colorimetric assay to quantitate total collagen levels.
• Summary
  • Detection method- Absorbance (OD = 560 nm). • Sample Types- Mammalian tissues, protein/peptide hydrolysates, serum, Urine • Applications- Measurement of collagen in various sample types.
• Detection Method
  Detection method- Absorbance (OD = 560 nm).
• Species Reactivity
  Mammalian tissues, protein/peptide hydrolysates, serum, urine
• Applications
  Measurement of collagen in various sample types.
• Sample Type
  • Mammalian tissues • Protein/peptide hydrolysates • Serum • Urine
• Features Benefits
  • Simple, fast and convenient assay • Can detect as low as 0.5 µg of collagen in a variety of samples
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Collagen is the most abundant insoluble protein found in the extracellular matrix and connective tissues. It can be found in skin, tendons, bone, cartilage, muscle, vitreous humor and ligaments, among other tissues. There are more than sixteen - well characterized types of collagens, but types I, II and III collagen comprise more than 80% content in mammals. The triple-helical structure of collagen is quite unique: it consists of a repeating pattern of a basic trimer: Glycine-Proline-Hydroxyproline. In cells, collagens are secreted as procollagens and these chains are transported into the Endoplasmic Reticulum, where, numerous post-translational modifications lead to the formation of a triple helix with disulfide bonds. Excessive production of collagen is linked to pathological conditions including liver cirrhosis, lung fibrosis, and tumor growth. BioVision’s Collagen Assay Kit is a simple and sensitive assay to detect small amounts of collagens in a variety of samples. The assay is based on the acid hydrolysis of samples to form hydrolysates and Hydroxyproline. This released Hydroxyproline gets oxidized to form a reaction intermediate, which further in the reaction, forms a chromophore (Abs 560 nm). The assay is simple, sensitive and specific for collagen and can detect as low as 0.5 µg of collagen in a variety of samples such as tissue homogenates, biological fluids and purified proteins.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Gene
  Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Collagen   Kit  
• Short name
  Collagen Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Total Collagen test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  total

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