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Products_type
Secondary Antibody
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Products_short_name
[IgG (H&L)]
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Products_name_syn
[Goat anti-Llama IgG (H&L) - Affinity Pure]
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Other_names
[immunoglobulin heavy chain variable region, partial]
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Reactivity
Llama. Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin llama serum immunoglobulins
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Specificity
Based on IEP, this antibody reacts with: heavy (gamma) chains on llama IgG, light chains on all llama immunoglobulins
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Purity
Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Llama IgG.
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Form
Clear, fluorescent yellow liquid
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Concentration
1.50 mg/ml (E 1% at 280 nm = 13.0)
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Storage_stability
Store at 2-8 degree C. Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Tested_application
Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
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Test
A high affinity purification column was use to purify Goat anti- IgG (H L) - Pure, FITC Conjugate by MyBioSource by chromatographic size exclusion.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure Goat anti- IgG (H L) - Pure, FITC Conjugate supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Anti llama or alpaca camelloid antibodies to detect the antibodies of the domesticated South American camelid, widely used as production animal with phage display of recombinant llama antibodies.
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Gene target
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Short name
Goat anti- IgG (H L) - Pure, FITC Conjugate
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Technique
affinity, anti-, anti, FITC, Goat, Pure, IgG, antibody to, antibodies, Fluorescein, goats, IgGs, pure products
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, Heavy & Light chains, IgG
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Label
FITC
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Species
Llama, Goats, Llamas
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Alternative name
caprine Antibody toLlama Immunoglobulin G (heavy + light chain) - protein Pure, fluorecein Conjugate
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Alternative technique
antibodies, purified, caprine, fluorescine, immunoglobulins
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