Anti-Caldesmon Antibody (SPM168)
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Catalog numberA1452-100
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Price:
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Size100 µg
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Antibody TargetCaldesmon
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Target Alternative NameCAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22
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Antibody TypeMonoclonal
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CloneSPM168
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Species ReactivitiesHuman
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Immunogen SequenceCrude human uterus extract
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AccessionQ05682
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Gene Id800
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AppearanceColorless liquid
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FormLiquid
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FormulationIn 10 mM PBS with 0.05% BSA & 0.05% azide
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PurificationProtein A/G purification
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ApplicationFC, IF, IHC
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Positive ControlIHC: human Uterus
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Application And UsagesFC: 0.5-1 ug/1X106 cells, IF: 1-2 ug/ml, IHC: 0.25-0.5 ug/ml
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DescriptionRecognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
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Storage Condition-20°C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedNot Verified
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Immunocytochemistry VerifiedVerified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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Additional descriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Short nameAnti-Caldesmon Antibody (SPM168)
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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IsotypeMouse IgG1, kappa
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Alternative nameAntibody toCaldesmon (antibody to-) (SPM168)
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data