E. coli 0157:H7 antibody FITC

• Catalog number
  60-E08
• Price
  Please ask
• Size
  500 µg

• Category
  Primary Antibody
• Antibody Subtype
  Conjugated Polyclonal Antibodies
• Area of research
  Infectious Disease
• Type of Immunogen
  E. coli 0157:H7 antibody (FITC) was raised in goat using whole cells of E. coli serotype 0157:H7 ..
• Raised in
  Goat
• Specificity
  E Coli 0157:H7
• Cross Reactivity
  Should be determined by the end user
• Conjugate Tag
  FITC
• Method of Purification
  E. coli 0157:H7 antibody (FITC) was purified by affinity chromatography.
• Form Buffer
  Affinity purified antibody conjugated to FITC and lyophilized with no preservatives added. Reconstitute with 1ml of laboratory grade water, dilute further with PBS or assay buffer.
• Storage
  Store at 4 deg C.
• Shipping conditions
  Blue Ice
• Tested for
  Please refer to the datasheet
• URL
• More info
• Properties
  If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  0157  
• Short name
  0157:H7 antibody FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Host
  e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. fitzgerald supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
• Label
  FITC
• Species
  E. coli, E. coli
• Alternative name
  Goat polyclonal E. coli 0157:H7 antibody (FITC) conjugated
• Alternative technique
  antibodies, escherichia, fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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