5’-Nucleotidase Activity Assay Kit (Colorimetric)

• Catalog number
  K992-100
• Price
  Please ask
• Size
  100 assays

• Description
  Simple, sensitive, enzymatic assay to measure 5'NT Activity in biological samples
• Summary
  • Detection method - Colorimetric (OD 570 nm) • Species reactivity - Mammalians • Applications - Measurement of 5’-Nucleotidase activity in various tissues/cells
• Detection Method
  Colorimetric (OD 670 nm)
• Species Reactivity
  Mammalian
• Applications
  • Measurement of 5’-Nucleotidase activity in various tissues/cells
• Features Benefits
  • Simple, highly sensitive, high-throughput compatible
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  5’-Nucleotidase (5’-NT), also known at CD73 (EC 3.1.3.5) is an enzyme located in the plasma membrane. It converts extracellular nucleotides like 5’-AMP to their corresponding nucleosides, through phosphorylitic cleavage. This conversion facilitates uptake of the nucleosides through nucleoside receptors into the cell, where they can again be phosphorylated to generate nucleotides and contribute to the nucleotide pool, inside the cell. 5’-NT levels are elevated in hepatic diseases such as viral hepatitis, alcoholic liver disease and cirrhosis. BioVision’s 5’-Nucleotidase Activity Kit is a simple two-step end point assay that relies on the Berthelot’s test for quantification of ammonia. In this assay, the action of 5’-nucleotidase on the substrate generates a product, which releases ammonia in presence of the converter. Developer I and II are then used to quantify the released ammonia through increase in absorbance at 670 nm. This assay can detect as low as 0.2 mU of 5’-NT. Since non-specific enzymes like alkaline phosphatase can give a positive signal in this assay, 5’-NT inhibitor may be used to completely inhibit 5’-nucleotidase and distinguish from the signal from non-specific enzymes. The assay kit also includes 5’- Nucleotidase (5’-NT) enzyme for use as positive control
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Nucleotidase   Activity   Kit  
• Short name
  5’-Nucleotidase Activity Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  5’-Nucleotidase Activity test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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