Mouse Anti-Mouse ADAM22 (cytoplasmic) Monoclonal IgG2B, Clone S46-30

• Catalog number
  SMC-411D-A680
• Price
  Please ask
• Size
  100 µg

• Clone
  S46-30
• Immunogen
  Fusion protein amino acids 757-857 (cytoplasmic region) of mouse ADAM22
• Antibody s full description
  Mouse Anti-Mouse ADAM22 Monoclonal IgG2B Antibody, Clone: S46-30: ATTO 680
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  MDC2 Antibody, Disintegrin and metalloproteinase domain-containing protein 22 Antibody, Metalloproteinase disintegrin ADAM22-3 Antibody, Metalloproteinase-like disintegrin-like and cysteine-rich protein 2 Antibody, ADAM 22 Antibody, ADAM metallopeptidase domain 22 Antibody, MGC149832 Antibody
• Raised in
  Mouse
• Antibody s target
  ADAM22
• Primary research fields
  Neuroscience, Cell Structure, Membrane Markers, Cell Signaling
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF, IP
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects the cytoplasmic domain of ADAM22 ~90kDa. Weak human detection.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  1 µg/ml of SMC-411 was sufficient for detection ofADAM22 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  ADAM 22 belongs to the ADAM gene family which havebeen shown to bind integrin and therefore may have a part in cell to cell or cell to matrix interactions. ADAM 22 is unique in the fact that it is only observed in the nervous system and predominantly in the brain. ADAM 22 is attached by cytoskeletal scaffolds to the postsynaptic density and is a receptor for LGI1.
• Bibliography
  1. Sagane K., Ohya Y., Hasegawa T., Tanaka I. (1998) Biochem J. 334 (Pt1): 93-98.
• Released date
  5-Feb-2013
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_001007221.1
• Gene number
  11496
• Protein number
  Q9R1V6
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ADAM22 (cytoplasmic) Monoclonal Antibody, Clone S46-30 (SMC-411). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ADAM22 (cytoplasmic) Monoclonal Antibody (SMC-411) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm. Magnification: 60X. Western Blot analysis of Rat brain membrane lysate showing detection of ADAM22 protein using Mouse Anti-ADAM22 Monoclonal Antibody, Clone S46-30 (SMC-411). Primary Antibody: Mouse Anti-ADAM22 Monoclonal Antibody (SMC-411) at 1:100. Mouse Anti-ADAM22 (cytoplasmic) Antibody [S46-30] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-411) Mouse Anti-ADAM22 Antibody [S46-30] used in Western Blot (WB) on Rat brain membrane lysate (SMC-411)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  ADAM22   cytoplasmic   Monoclonal   IgG2B   S46-30  
• Gene symbol
  ADAM22, SNORD114-30, SNORD116-30, SNORD115-30, IGKV2-30, RNU6-30P, IGHV3-30, IGLV3-30, IGHVII-30-21, IGHVII-30-1
• Short name
  Mouse Anti-Mouse ADAM22 (cytoplasmic) Monoclonal IgG2B, Clone S46-30
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG2B, IgG2b
• Label
  ATTO 680
• Species
  Mouse, Mouses
• Alternative name
  Mouse Antibody toMouse ADAM metallopeptidase domain 22 (cytoplasmic) monoclonal IgG2B, clonality S46-30
• Alternative technique
  murine, antibodies
• Alternative to gene target
  ADAM metallopeptidase domain 22, ADAM22 and IDBG-25323 and ENSG00000008277 and 53616, zinc ion binding, Plasma membranes, Adam22 and IDBG-133249 and ENSMUSG00000040537 and 11496, ADAM22 and IDBG-633802 and ENSBTAG00000007136 and 523645
• Clone name
  S46-30

Gene info

• Identity
  HGNC:201
• Gene
  ADAM22
• Long gene name
  ADAM metallopeptidase domain 22
• Synonyms gene name
  • a disintegrin and metalloproteinase domain 22
• Synonyms
  • MDC2
• Synonyms name
  • metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2
• GenBank acession
  • AB009671
• Locus
  7q21.12
• Discovery year
  1998-12-01
• Entrez gene record
  53616
• Pubmed identfication
  • 9693107
  • 10524237
• RefSeq identity
  • NM_021723
• Classification
  • ADAM metallopeptidase domain containing
• VEGA ID
  OTTHUMG00000137417

Gene info

• Identity
  HGNC:33018
• Gene
  SNORD114-30
• Long gene name
  small nucleolar RNA, C/D box 114-30
• Synonyms
  • 14q(II-30)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767611
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003223.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:42797
• Gene
  SNORD116-30
• Long gene name
  small nucleolar RNA, C/D box 116-30
• Synonyms
  • HBII-85-30
• Locus
  15q11.2
• Discovery year
  2011-08-22
• Entrez gene record
  100873856
• RefSeq identity
  • NR_047032
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33049
• Gene
  SNORD115-30
• Long gene name
  small nucleolar RNA, C/D box 115-30
• Synonyms
  • HBII-52-30
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033804
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003345.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5785
• Gene
  IGKV2-30
• Long gene name
  immunoglobulin kappa variable 2-30
• GenBank acession
  • X63403
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28919
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151692

Gene info

• Identity
  HGNC:34274
• Gene
  RNU6-30P
• Long gene name
  RNA, U6 small nuclear 30, pseudogene
• Synonyms gene
  • RNU6-30
• Synonyms gene name
  • RNA, U6 small nuclear 30
• Locus
  Xq22.1
• Discovery year
  2008-06-10
• Entrez gene record
  100873747
• RefSeq identity
  • NR_046490

Gene info

• Identity
  HGNC:5591
• Gene
  IGHV3-30
• Long gene name
  immunoglobulin heavy variable 3-30
• GenBank acession
  • M83134
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28439
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000188332

Gene info

• Identity
  HGNC:5912
• Gene
  IGLV3-30
• Long gene name
  immunoglobulin lambda variable 3-30 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable 3-30
  • immunoglobulin lambda variable 3-30 pseudogene
• GenBank acession
  • Z73646
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28789
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151182

Gene info

• Identity
  HGNC:52400
• Gene
  IGHVII-30-21
• Long gene name
  immunoglobulin heavy variable (II)-30-21 (pseudogene)
• Synonyms
  • IGHV(II)-30-21
• Locus
  14q32.33
• Discovery year
  2016-11-10
• Entrez gene record
  107548099
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33

Gene info

• Identity
  HGNC:5676
• Gene
  IGHVII-30-1
• Long gene name
  immunoglobulin heavy variable (II)-30-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-30-1
  • immunoglobulin heavy variable (II)-30-1 pseudogene
• Synonyms
  • IGHV(II)-30-1
• GenBank acession
  • AB019439
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28371
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152099

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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