Caspase-10/b, human recombinant
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Catalog number1090B-25
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PricePlease ask
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Size25 units
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SynonymsCaspase-10/b
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Alternative_namesCaspase-10, CASP-10, Apoptotic protease Mch-4, FAS-associated death domain protein interleukin-1B-converting enzyme 2, FLICE2, ICE-like apoptotic protease 4
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DescriptionA cysteine protease involved in apoptosis
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RecombinantYes
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SourceE. coli.
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Purity by SDS PAGE≥90%
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AssaySDS-PAGE
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Activity Specifications test method> 15000 units/mg
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Unit DefinitionOne unit of the recombinant caspase-10/b is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
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Molecular Weighttwo large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form.
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Storage Temp-70°C
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Shippinggel pack
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Shelf Life12 months
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AppearanceLyophilized powder
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Physical form descriptionLyophilized powder
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Reconstitution InstructionsReconstitute to 1 unit per ul in PBS containing 15% glycerol.
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Background InformationCaspase-10b (also known as FLICE 2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-10 also exists in cells as an inactive proenzyme. During apoptosis procaspase-10 is processed at aspartate residues to form active caspase-10 which consists of two large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form. The recombinant active human caspase-10/b was expressed in E. coli. The active caspase-10 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Additional descriptionHuman and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolBCL10
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Short nameCaspase-10/b, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative namecaspase-10/b, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameBCL10 immune signaling adaptor
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Synonyms gene name
- B cell CLL/lymphoma 10
- BCL10, immune signaling adaptor
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-01-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CBM complex
- Caspase recruitment domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data