PicoProbe™ D-2-Hydroxyglutarate Dehydrogenase Assay Kit (Fluorometric)
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Catalog numberK248-100
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Price:
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Size100 assasys
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DescriptionRapid, sensitive & simple assay to measures D-2Hydroxyglutarate Dehydrogenase activity
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Summary• Detection method- Fluorescence (Ex/Em=535/587 nm) • Application- Detection of D-2-Hydroxyglutarate Dehydrogenase activity
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Detection MethodFluorescence (Ex/Em=535/587 nm)
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Species ReactivityMammalian
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ApplicationsDetection of D-2-Hydroxyglutarate Dehydrogenase activity
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Sample Type• Purified recombinant protein • Cell and tissue lysate
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Features Benefits• Rapid, sensitive and convenient • The kit measures total Activity of D-2-Hydroxyglutatare Dehydrogenase Activity with limit of quantification 4 µU/µl using recombinant D2HGDH
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundD-2-Hydroxyglutarate Dehydrogenase (D2HGDH: EC 1.1.99.2) is a mitochondrial enzyme which catalyzes the conversion of D-2-Hydroxyglutarate into 2-Oxoglutarate. In humans, mutation of D2HGDH gene could reduce D2HGDH activity which will cause the D-2-Hydroxyglutarate accumulation in body fluids. Increased D-2-Hydroxyglutarate level in body fluids leads to D-2-Hydroxyglutaric Aciduria, a neurometabolic disorder characterized as developmental delay, epilepsy, hypotonia, and dysmorphic features. BioVision’s PicoProbe™ D-2-Hydroxyglutarate Dehydrogenase Assay kit provides a quick and easy method for monitoring D2HGDH activity in a wide variety of samples. In this assay, D2HGDH converts D-2-Hydroxyglutarate into 2-Oxoglutarate and NADH, which further reduces PicoProbe™ to generate an intense fluorescent product (Ex/Em = 535/587 nm). This kit is simple, sensitive and high-throughput adaptable and can detect as low as 4 µU/µl of D2HGDH activity.
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TestBiovision supplies other types of Assays as 1.
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ConjugatePicoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit
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Gene target
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Gene symbolRAB3D, NCAPD2, HMGN2P46, DDT, SNAR-D, PDPN, DGCR11, IGHD2-15
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Short name™ D-2-Hydroxyglutarate Dehydrogenase Assay Kit (Fluorometric)
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TechniqueAssay, assays
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HostAssay
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Alternative namePicoProbe™ D-2-Hydroxyglutarate Dehydrogenase test reagent (fluorescence)
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Alternative techniquearrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameRAB3D, member RAS oncogene family
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-06-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RAB, member RAS oncogene GTPases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenon-SMC condensin I complex subunit D2
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Synonyms gene name
- non-SMC condensin I complex, subunit D2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-09-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Condensin I subunits
- Armadillo like helical domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namehigh mobility group nucleosomal binding domain 2 pseudogene 46
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Synonyms gene
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Synonyms gene name
- chromosome 15 open reading frame 21
- high-mobility group nucleosomal binding domain 2 pseudogene 46
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-02-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameD-dopachrome tautomerase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-03-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall NF90 (ILF3) associated RNA D
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Synonyms gene name
- small ILF3/NF90-associated RNA D
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GenBank acession
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Locus
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Discovery year2008-07-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small NF90 (ILF3) associated RNAs
Gene info
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Identity
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Gene
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Long gene namepodoplanin
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-07-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameDiGeorge syndrome critical region gene 11
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Synonyms gene name
- DiGeorge syndrome critical region gene 11
- DiGeorge syndrome critical region gene 11 (non-protein coding)
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-11-13
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Entrez gene record
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Pubmed identfication
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Classification
- Long non-coding RNAs with non-systematic symbols
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy diversity 2-15
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID