Prohibitin Antibody
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Catalog number3805-100
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PricePlease ask
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Size100 µg
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Antibody TargetProhibitin
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Target Alternative NamePHB, phb
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic peptide surrounding amino acid 256 of human Prohibitin
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AccessionP35232
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Gene Id5245
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot, Immunoprecipitation, Immunofluorescence
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Application And UsagesWestern blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml) and Immunofluorescence (5-10 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 33 kDa protein, corresponding to the apparent molecular mass of Prohibitin on SDS-PAGE immunoblots, in samples from human, mouse and rat origins.
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DescriptionProhibitin is an evolutionarily conserved protein that has anti-proliferative activity. Prohibitin is a 30 kDa, post-synthetically modified protein found in the inner membrane of mitochondria and on the plasma membrane of B cells. It mediates B cell mutation. Prohibitin Controls cell cycle in both transcriptional and post-translational mechanisms. Prohibitin is a potential tumor suppressor. Prohibitin gene mutations are correlated with the breast cancer development and/or progression in over 80%0f the cell lines analyzed.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolPHBP5, PHBP14, PHBP18
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Short nameProhibitin Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameProhibitin (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameprohibitin pseudogene 5
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Locus
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Discovery year2010-11-26
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprohibitin pseudogene 14
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Locus
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Discovery year2010-11-26
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprohibitin pseudogene 18
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Locus
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Discovery year2015-03-05
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Entrez gene record
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data