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C5a His Tag human recombinant

C5a His Tag human recombinant is available 3 times from Biovision labs

4995A-100 | C5a (His-Tag), human recombinant size: 100 ug | 796.35 USD

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4995A-1000 | C5a (His-Tag), human recombinant size: 1 mg | 5,881.48 USD

Catalog number 4995A-1000
Supplier Biovision
Price 5,881.48 USD
Size 1 mg
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    #Human#.jpg#0#.jpegC5a (His-Tag), human recombinant

4995A-20 | C5a (His-Tag), human recombinant size: 20 ug | 302.53 USD

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Synonyms Recombinant human C5a (His-Tag)
Alternative_names C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin chemotactic receptor (C5a-R), (C5aR,) (CD antigen CD88), C5AR1, C5AR, C5R1
Description A potent chemotactic factor for human peripheral blood neutrophils and monocytes
Recombinant Yes
Source E. coli
Purity by SDS-PAGE ≥95%
Assay SDS-PAGE
Biological activity Human recombinant C5a is as active as that of serum derived C5a based on enzyme release assay of myeloperoxidase. The current lot of ED₅₀ = 1.36 nmolar.
Molecular Weight (74 amino acids)
Storage Temp. -20°C
Shipping Gel pack
Shelf Life 12 months
Appearance Lyophilized protein
Physical form description Lyophilized with no additives
Reconstitution Instructions Reconstitute to a concentration of 1.0 mg/ml in ice-cold H₂O containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C for future use. Frozen/thaw will result in some loss of activity.
Background Information Human complement C5a is a 74-amino acid glycopeptide generated during complement activation from the a-chain of complement C5. C5a is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. The 74 amino acid, human C5a protein expressed in E. coli with a 6 amino acid, N-terminal His·Tag sequence and 28 amino acid proprietary vector sequence necessary for expression (C5a without His-tag is alailable, BioVision Cat # 4995B). The recombinant protein is not further processed to remove the additional sequence. Recombinant C5a has an activity similar to that of serum derived C5a based on enzyme assay of myeloperoxidase release.
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Conjugation histidine
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Gene targetC5a His-Tag
Short name C5a (His-Tag) recombinant
Technique Recombinant
Label His
Species Human
Alternative name C5a (histidine-detection labelled), H. sapiens recombinant
Alternative technique rec
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C5a (His-Tag) recombinant | Technique alternative | 01012195
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