Dog clusterin,CLU ELISA Kit

  • Catalog number
    EK0054
  • Price
    Please ask
  • Size
    96-wells plate (breakable strips)
  • Long name
    ELISA test kit for Dog clusterin,CLU
  • Synonym name
    Dog clusterin,CLU ELISA Kit
  • General description
    This is a ready to use kit for performing ELISA tests for detection of clusterin,CLU in samples from Dog
  • Antigen
    clusterin,CLU
  • Species reactivity
    Dog
  • Sample types
    Our clusterin,CLU ELISA kit has been proven to work and successfully detect clusterin,CLU in Canine
  • Kit contents
    ELISA test kit for Dog clusterin,CLUAssay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
  • Detection wavelengt
    450 nm
  • Assay procedure
    1-4h
  • Summary of the prodecure
    1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
  • Applications
    Enzyme Linked Immunosorbent Assay
  • Shipping storage shelf life
    The clusterin,CLU ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Dog clusterin,CLU ELISA Kit is 6 months after delivery.
  • Tips
    Due to transportation and handling small amounts of the liquid components of the Dog clusterin,CLU ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
  • Properties
    Canine or Canis Lupus is mostly Beagle used for drug research. SAB ELISA KIT produces ELISA test kits and polyclonal antibodies. E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Gene target
    clusterin   CLU  
  • Gene symbol
    CLU
  • Short name
    clusterin,CLU ELISA
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Dog, Dogs
  • Alternative name
    canine clusterin,clusterin Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas, kits
  • Alternative to gene target
    clusterin, APO-J and APOJ and CLI and CLU1 and CLU2 and KUB1 and NA1/NA2 and SGP-2 and SGP2 and SP-40 and TRPM-2 and TRPM2, CLU and IDBG-14287 and ENSG00000120885 and 1191, misfolded protein binding, nuclei, Clu and IDBG-174736 and ENSMUSG00000022037 and 12759
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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