Beta-lactaminase, Human Recombinant
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Catalog number
P1196-100
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Price
Please ask
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Size
100 ug
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Synonyms
ampC, ampA, Cephalosporinase
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Alternative_names
ampC, ampA, Cephalosporinase
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Description
Serine beta-lactamase with a substrate specificity for cephalosporins
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Recombinant
Yes
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Source
E.coli
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Purity by SDS PAGE
> 95%
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Assay
SDS-PAGE
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Activity Specifications test method
Measured by its ability to hydrolyze 1.0 µmole of Nitrocefin per minute at pH 7.0 at 37C.
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Biological activity
Specific activity is >700 units/mg
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Results
>700 units/mg
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Unit Definition
One unit will hydrolyze 1.0 µmole of Nitrocefin per minute at pH 7.0 at 37C.
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Molecular Weight
41.8 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Liquid
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Physical form description
In 20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol
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Background Information
AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Recombinant E.coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
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Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Beta-lactaminase, Recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
b-lactaminase, H. sapiens Rec.
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Alternative technique
rec
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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