Protein A/G/L Sepharose Column
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Catalog number
6548-5
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Price
Please ask
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Size
5ml
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Description
Columns with Reusable Protein A/G/L conjugated Sepharose beads with ≥10 mg/ml binding capacity & high flow rate for antibody purification from multiple sources. Protein A/G/L-Sepharose is prepared by covalently coupling recombinant Protein A/G/L (Cat.# 6540; containing five Ig-binding regions of protein L, five IgG binding domains from Protein A , and three Ig-binding regions of protein G. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure the maximum specific IgG binding) to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity. The capacity of IgG binding could be greater than 10 mg of human IgG per ml of wet bead.
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Product Highlights
• CONTENTS: Ready-to-use pre-packed columns of 1 ml or 5 ml bead volume in PBS with 0.02% sodium azide. • FEATURES: Protein A/G/L binds to all IgG subclasses from various mammalian species, including all IgGs that bind to Protein A, Protein G, and Protein L, individually, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies. The binding capacity is greater than 10 mg/ml of bead; High flow rate; Low falling off of rProtein A/G/L; pH stability 2-10. • APPLICATIONS: Purification of monoclonal and polyclonal antibodies.
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Storage Temp
+4°C
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Shipping
gel pack
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Shelf Life
12 months
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Notes
For research use only. Not to be used for human or animal treatment or consumption.
MeSH Data
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Name
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Concept
Scope note:
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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