Recombinant Human LILRB4 / CD85k / ILT3 Protein (His Tag)
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Catalog number
PKSH030439-20µg
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Price
Please ask
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Size
20µg
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Synonym
CD85K;ILT-3;ILT3;LIR-5;LIR5
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Activity
NA
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Sequence
Met1-Glu259
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Fusion tag
C-His
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Accession
AAH26309.1
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Expressed Host
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
＞95 % as determined by SDS-PAGE.
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Endotoxin
＜1.0 EU per µg as determined by the LAL method.
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
27.7 kDa
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AP Mol Mass
27.7 kDa
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Formulation
Lyophilized from sterile PBS, pH 7.4
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Background
Mouse Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4 (LILRB4/CD85k/ILT3) is an approximately transmembrane glycoprotein that negatively regulates immune cell activation. Mouse LILRB4 consists of a 215 amino acid (aa) extracellular domain with two Ig-like domains, a 22 aa transmembrane segment, and a 75 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM). Within the ECD, mouse LILRB4 shares 45% and 77% aa sequence identity with human and rat LILRB4, respectively. Alternative splicing of mouse LILRB4 generates a potentially soluble isoform that lacks the transmembrane segment. LILRB4 is expressed on dendritic cells (DC), monocytes, macrophages, and vascular endothelial cells (EC). Ligation of LILRB4 triggers ITIM-mediated inhibition of cellactivating signaling, leading to enhanced immune tolerance and reduced allogeneic graft rejection. Soluble LILRB4 induces the differentiation of CD8+ T suppressor cells (Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL. In turn, CD8+ Ts cells induce LILRB4 up-regulation and a tolerogenic phenotype in monocytes, DC, and EC.
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Conjugation
histidine
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
LILRB4
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Short name
Recombinant LILRB4 / CD85k / ILT3 Protein (His Tag)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
His
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Species
Human, Humans
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Alternative name
Rec. H. sapiens leukocyte immunoglobulin-like receptor, subfamily B (including TM and ITIM domains), member 4 / CD85k / ILT3 Protein (histidine detection labelled)
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Alternative technique
rec
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Alternative to gene target
leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4, CD85K and ILT-3 and ILT3 and LIR-5 and LIR5, LILRB4 and IDBG-68893 and ENSG00000186818 and 11006, protein binding, Plasma membranes
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Gene info
Product images
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