pLL-EF1a-GFP-T2A-Puro [Lenti-LabelerTM virus]

Shipping Temperature

Dry Ice
Description

Lenti kit or vector with CMV or EF promotors for producing Lentivirus (lenti-, Latin for "slow") or lentiviral particles in 293t cells. Lentiviruses are characterized by a long incubation period and often Puro selected. Lentiviruses can deliver a significant amount of viral RNA into the DNA of the host cell and have the unique ability among retroviruses of being able to infect non-dividing cells. They are one of the most efficient methods of a gene delivery vector. The are packaged in HEK 293T cells to get the highest possible lentiviral titers of 108 IFU/ml. The Luc reporter is the expression of the firefly luciferase gene. Puro stands for puromicin selection.

Gene target

pLL-EF1a-GFP-T2A-Puro LabelerTM virus]
Short name

pLL-EF1a-GFP-T2A-Puro [ -LabelerTM virus]
Technique

lenti, Lentivectors and viral particles
Label

gfp
Species

Virus, Viruses
Alternative name

pLL-EF1a-Green Fluorescent-T2A-Puro [lentiviral-LabelerTM virus]
Virus

lenti

Name

Artificial Gene Fusion
Concept

Scope note: The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses.
Tree numbers
- E05.393.220.250
Qualifiers

drug effects, methods, radiation effects