Human CellExp™ CD28, Human recombinant
-
Catalog number
9237-10
-
Price
Please ask
-
Size
10 µg
-
-
Synonyms
CD28, Tp44
-
Alternative_names
CD28, Tp44
-
Description
T-cell-specific surface glycoprotein CD28
-
Recombinant
Yes
-
Source
HEK 293 cells
-
Purity by SDS PAGE
≥92%
-
Assay
SDS-PAGE
-
Purity
≥92%
-
Endotoxin Level
< 1 EU/ug
-
Activity Specifications test method
Measured by its binding ability in a functional ELISA.
-
Biological activity
Immobilized human CD28 at 2 ug/mL (100 uL/well) can bind human Biotin-B7-1 (CD80) Fc with a linear range of 0.01-0.5 ug/mL when detected by Streptavidin-HRP second antibody.
-
Binding Capacity
Immobilized Human B7-1, His Tag at 20ug/ml, can bind Human CD28, Fc Tag with a linear of 0.2-20 ug/ml and Human B7-2, His Tag at 2ug/ml, can bind Human CD28, Fc Tag with a linear of 0.01-5 ug/ml
-
Molecular Weight
This protein is fused with a Fc tag at C-terminus and has a calculated MW of 41.8 kDa.
-
Storage Temp
-20°C
-
Shipping
Gel pack
-
Shelf Life
12 months
-
Appearance
Lyophilized
-
Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4.
-
Reconstitution Instructions
Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml
-
Background Information
T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
CD28
-
Short name
CellExp™ CD28, recombinant
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Species
Humans
-
Alternative name
H. sapiens CellExp™ CD28 molecule, H. sapiens Rec.
-
Alternative technique
rec
-
Alternative to gene target
CD28 molecule, Tp44, CD28 and IDBG-79183 and ENSG00000178562 and 940, identical protein binding, Cell surfaces, Cd28 and IDBG-159608 and ENSMUSG00000026012 and 12487, CD28 and IDBG-643371 and ENSBTAG00000007445 and 281050
-
Gene info
-
Identity
-
Gene
-
Long gene name
CD28 molecule
-
Synonyms gene name
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1988-05-11
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- CD molecules
- Ig-like cell adhesion molecule family
- V-set domain containing
-
VEGA ID
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
