96-Well ROCK Activity Assay test kit
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Catalog number
STA-416
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Price
Please ask
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Size
96 assays
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We send this reagant on
Blue Ice
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The temperatureerature for storage is
Store active ROCK-II at -80ºC, ATP Solution at -20ºC and all other test kit components at 4ºC. Avoid multiple freeze/thaw cycles.
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SDS or safty data sheet
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Type of reagent
Cell Signaling and Protein Biology
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Related techniques
Cell Signaling and Protein Biology, Kinase Assays, Rho Kinase (ROCK) Assays
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Use of the reagent
Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay test kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The test kit contains a strip-well plate pre-coated with recombinant MYPT1.
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Cellular use
Safe non-radioactive ELISA format
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Protocol information
Active ROCK-II included as positive control
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Living modified organisme in this reagent or LMO
Recombinant Protein from Baculovirus
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Species compatible to
Humans
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Use of this test kit or product
Genetics lab
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Antigen
a specific protein sequence
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,1
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Molecular weight
in kilo Danlto or kDa
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This reagent is purified
0.97
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Test
Cellular biology labs supplies other types of Assays as 1.
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Gene target
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Short name
96- ROCK Activity Assay test kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
96-Well ROCK Activity test test reagent
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Alternative technique
arrays, tests, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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