Caspase-3 Activity Assay Kit II (Fluorometric)

• Catalog number
  K533-100
• Price
  Please ask
• Size
  100 assays

• Description
  A sensitive assay kit for the quantification of caspase 3 activity in mammalian cell culture. Standard and Positive Control included.
• Detection Method
  Fluorometric (Ex/Em =400/505 nm)
• Species Reactivity
  Mammalian
• Applications
  Comparison between apoptotic sample an uninduced control; allows determination of the fold increase in caspase-3/CPP32 activity
• Sample Type
  Cell and tissue culture supernatants,Cell lysate
• Features Benefits
  Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.,Fluorometric (400/505 nm),Simple, 1-Step Procedure; takes 1-2 hours to complete,Complete kit. Includes positive control and AFC as standard
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Caspase-3 (3.4.22.56) is a cysteine protease that cleaves the C-terminal of an Aspartate residue at the P1 position. The cleavage event is a critical step in the downstream signaling and amplification of apoptosis, or programmed cell death. Apoptosis is a tightly regulated event that plays a central role in the development and homeostasis of multi-cellular organisms. BioVision’s Caspase-3 Fluorometric Activity Assay Kit II allows the detection and quantification of Caspase-3 Activity by using a synthetic substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin), which upon cleavage by Caspase-3, will emit a strong, stable fluorometric signal (Ex/Em= 400/505 nm). The assay is sensitive, fast and allows for the high-throughput quantification of Caspase-3 Activity in cell lysate or adherent cells and can be used to evaluate induction of apoptosis and/or evaluation of apoptosis inhibitors. As little as 11.25 mU of activity per well and less than 500 apoptotic cells can be detected
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

• Gene target
  Caspase-3   Activity   Kit   Fluorometric  
• Short name
  Caspase-3 Activity Assay Kit II (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  caspase-3 Activity test reagent II (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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