This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
In PBS with 0.05% (W/V) sodium azide.
Alternative names of antibody target:
PEBP2B, CBF-beta, PEA2-beta, PEB2-beta
ChIP: 4 µl/ChIP, ELISA: 1:500.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Clonality of antibody:
Polyclonal antibody raised in rabbit against human CBFb (core-binding factor, beta subunit) using two KLH conjugated synthetic peptides containing sequences from the central region of the protein.
How to handle this antibody:
The antibody solution should be gently mixed before use.
CBFb represents the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family. These transcription factors regulate a host of genes specific to hematopoiesis (e.g. RUNX1) and osteogenesis (e.g. RUNX2). The beta subunit is the regulatory subunit which allosterically enhances the activity of the DNA binding alpha subunit as the complex binds to the core site of various enhancers and promoters. CBFb can be involved in a chromosomal rearrangement of chromosome 16 (inv(16)(p13q22)) which produces a fusion protein consisting of the N terminus of CBFb and the C-terminal portion of MYH11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
anti-CBFb polyclonal antibody
Polyclonal, Antibody, anti
antibody to-CBFb polyclonal (Antibody to)