CBFb polyclonal antibody
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Catalog number6835-50
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PricePlease ask
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Size50 μl
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Antibody TargetCBFb
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Target Alternative NamePEBP2B, CBF-beta, PEA2-beta, PEB2-beta
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Antibody TypePolyclonal
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Species ReactivitiesHuman.
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Immunogen SequencePolyclonal antibody raised in rabbit against human CBFb (core-binding factor, beta subunit) using two KLH conjugated synthetic peptides containing sequences from the central region of the protein.
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AccessionQ13951
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Gene Id865
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AppearanceColorless liquid
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FormLiquid
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FormulationIn PBS with 0.05% (W/V) sodium azide.
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PurificationWhole Anti-Sera
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ApplicationELISA, ChIP.
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Positive ControlELISA: Peptides, ChIP: SKNO-1 cells.
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Application And UsagesChIP: 4 µl/ChIP, ELISA: 1:500.
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DescriptionCBFb represents the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family. These transcription factors regulate a host of genes specific to hematopoiesis (e.g. RUNX1) and osteogenesis (e.g. RUNX2). The beta subunit is the regulatory subunit which allosterically enhances the activity of the DNA binding alpha subunit as the complex binds to the core site of various enhancers and promoters. CBFb can be involved in a chromosomal rearrangement of chromosome 16 (inv(16)(p13q22)) which produces a fusion protein consisting of the N terminus of CBFb and the C-terminal portion of MYH11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedNot Verified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedVerified
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ChIP VerifiedVerified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translationanticorps
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Gene target
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Gene symbolCBFB
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Short nameCBFb polyclonal antibody
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TechniquePolyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeRabbit IgG
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Alternative nameCBFb polyclonal (antibody to-)
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene namecore-binding factor subunit beta
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Synonyms gene name
- core-binding factor, beta subunit
- core-binding factor beta subunit
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data