This antibody has been succesfully tested for use in following methods: ELISA, ChIP.
Target of this antibody: CBFb
Formulation of this antibody: In PBS with 0.05% (W/V) sodium azide.
Alternative names of antibody target: PEBP2B, CBF-beta, PEA2-beta, PEB2-beta
Recommended usage: ChIP: 4 µl/ChIP, ELISA: 1:500.
Storage information: Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions: gel pack
Antibody host: Rabbit
This antibody reacts with: Human.
Clonality of antibody: Polyclonal
Isotype: Rabbit IgG
Purification method: Whole Anti-Sera
Immunogene sequence: Polyclonal antibody raised in rabbit against human CBFb (core-binding factor, beta subunit) using two KLH conjugated synthetic peptides containing sequences from the central region of the protein.
How to handle this antibody: The antibody solution should be gently mixed before use.
Background information: CBFb represents the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family. These transcription factors regulate a host of genes specific to hematopoiesis (e.g. RUNX1) and osteogenesis (e.g. RUNX2). The beta subunit is the regulatory subunit which allosterically enhances the activity of the DNA binding alpha subunit as the complex binds to the core site of various enhancers and promoters. CBFb can be involved in a chromosomal rearrangement of chromosome 16 (inv(16)(p13q22)) which produces a fusion protein consisting of the N terminus of CBFb and the C-terminal portion of MYH11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype.
Form of antibody: Liquid
Concentration of antibody: N/A
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation anticorps
Short name anti-CBFb polyclonal antibody
Technique Polyclonal, Antibody, anti
Alternative name antibody to-CBFb polyclonal (Antibody to)
Alternative technique polyclonals