Phospho-p53 Antibody

• Catalog number
  3515-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Phospho-p53
• Target Alternative Name
  TP53 , p53 , LFS1 , TRP53 , P53 , tumor protein p53 , Li-Fraumeni syndrome
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, Rat
• Immunogen Sequence
  KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human p53.
• Accession
  EAW90140
• Gene Id
  7157
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  1 mg/ml
• Formulation
  In 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.
• Purification
  Affinity purified
• Application
  WB, IHC, IF
• Positive Control
  WB: Hela UV-treated; PC12 whole cell lysates; IHC: human breast cancer; IF: PC12 cells
• Application And Usages
  WB 1:500 – 1:1000; IHC 1:100 – 1:200; IF/IC 1:100 – 1:500; IP 1:10 – 1:100. However, the optimal conditions should be determined individually
• Description
  The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. p53 can apparently be phosphorylated by ATM, ATR, and DNA-PK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Phospho-p53  
• Short name
  Phospho-p53 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  phosphorilated-p53 (antibody to-)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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