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Target
p53 (pSer37)
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Conjugate
FITC
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Research Area s
Cancer, Cell Cycle, p53 Pathway, Cancer, Tumor suppressors, Apoptosis, Cell Signaling, Epigenetics and Nuclear Signaling, DNA/RNA, DNA Damage and Repair
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Alternative Name s
p53 tumor suppressor Antibody, Cellular tumor antigen p53 Antibody, Antigen NY-CO-13 Antibody, Phosphoprotein p53 Antibody, Tumor protein 53 Antibody, BCC7 Antibody, p53 Antibody, TP53 Antibody, P53_HUMAN Antibody, FLJ92943 Antibody, Mutant tumor protein 53 Antibody, Tumor protein p53 Antibody, LFS1 Antibody, Transformation related protein 53 Antibody, TRP53 Antibody, Tumor suppressor p53 Antibody
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Category
Antibodies
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Product Type
Polyclonal
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Immunogen
Synthetic phospho-peptide from human p53 around the phosphorylation site of serine 37 (pSer37).
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Immunogen Species
Human
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Accession Number
NP_000537.3
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Gene ID
7157
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Swiss Prot
P04637
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Applications
WB, IHC, ELISA
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Host Species
Rabbit
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Species Reactivity
Human
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Antibody Dilution
WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000); optimal dilutions for assays should be determined by the user.
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Purification
Peptide Affinity Purified
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Storage Buffer
PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide
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Specificity
Detects endogenous levels of p53 protein only when phosphorylated at serine 37 (pSer37). Detects a band at ~44 kDa.
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Storage Temperature
-20ºC
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Shipping Temperature
Blue Ice or 4ºC
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Cellular Localization
Mitochondrion Matrix, Endoplasmic Reticulum, Nucleus Pml Body, Nucleus, Pml Body, Cytoplasm, Cytosol, Mitochondrion, Nuclear Chromatin, Nuclear Matrix, Nucleolus, Nucleoplasm, Intracellular, Protein Complex, Replication Fork
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Field of Use
For in vitro research use only.
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Gross Weight kg
1.4
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Net Weight g
0.1
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Properties
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
