Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric)
-
Catalog number
K767-100
-
Price
Please ask
-
Size
100 assays
-
-
Description
The most sensitive kit on the market
-
Summary
• Detection: Fluorescence (Ex/Em = 535/587 nm) • Application: Detection of Purine Nucleoside Phosphorylase activity in variety of samples
-
Detection Method
Fluorescence (Ex/Em = 535/587 nm)
-
Applications
Detection of Purine Nucleoside Phosphorylase activity in variety of samples
-
Sample Type
• Purified recombinant protein • Cell and tissue lysate
-
Features Benefits
• Rapid, simple & convenient • Limit of quantification is 0.005 µU recombinant Purine Nucleoside Phosphorylase
-
Storage Conditions
-20°C
-
Shipping Conditions
gel pack
-
Shelf life
12 months
-
Background
Purine Nucleoside Phosphorylase (PNP) (E.C. 2.4.2.1.) is an enzyme involved in purine metabolism and it catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides, in the presence of inorganic phosphate as a second substrate, to generate the purine base and ribose-1-phosphate or deoxyribose-1-phosphate. It is one of the enzymes of the nucleotide salvage pathways that allows the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). PNP is a cytosolic enzyme. PNP deficiency disease leads to toxic buildup of deoxyguanosine in T-cells leading to T-lymphocytopenia with no apparent effects on B-lymphocyte function. Inhibition of PNP is an important target for chemotherapeutic applications and treatment of T- cell mediated autoimmune diseases. PNP deficiency is also associated with neurological problems. In BioVision’s Purine Nucleoside Phosphorylase Activity Assay, hypoxanthine formed from the breakdown of inosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the PNP Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification is 0.005 µU recombinant Purine Nucleoside Phosphorylase.
-
-
Test
Biovision supplies other types of Assays as 1.
-
Gene target
-
Short name
Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric)
-
Technique
Assay, assays
-
Host
Assay
-
Alternative name
Purine Nucleoside Phosphorylase Activity test reagent (fluorescence)
-
Alternative technique
arrays, kits
-
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
-
MeSH Data
-
Name
-
Concept
Scope note:
Methods or procedures used to obtain samples of URINE.
-
Tree numbers
- E01.370.225.998.762
- E05.200.998.762
-
Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
Product images
Similar products