Taq DNA Polymerase Polyclonal Antibody
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Catalog number
7018-100
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Price
Please ask
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Size
100 µg
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Antibody Target
Taq DNA Polymerase
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Target Alternative Name
DNA polymerease
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Antibody Type
Polyclonal
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Immunogen Sequence
Recombinant Taq DNA Polymerase (Cat No. 9001)
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Accession
B6VA24
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
0.5 mg/ml
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Formulation
100 µg (0.5 mg/ml) of antibody in PBS pH 7.2, 0.01 % BSA, 0.03 % ProClin®, and 50 % glycerol.
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Purification
Affinity purified rabbit IgG
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Application
Western blot
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Positive Control
Western blot: DNA polymerase
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Application And Usages
Western blot: 1-4 µg/ml
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Description
Taq DNA Polymerase is a thermostable enzyme that replicates DNA and exhibits a half-life of 40 minutes at 95°C. Taq catalyzes the polymerization of nucleotides into duplex DNA in the 5’ –3’ direction in the presence of magnesium. The enzyme has an apparent molecular weight of 94,000 daltons by SDS-PAGE and exhibits 5’– 3’exonuclease activity.
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
Taq DNA Polymerase Polyclonal Antibody
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Technique
Polyclonal, Antibody, dna, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. Biovision TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
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Isotype
rabbit IgG
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Alternative name
Taq Desoxyribonucleic acid polymerizing enzyme polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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