Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3/UCH-L3 (C-6His)
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Catalog numberC189-1000
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Price:
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Size1 mg
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DescriptionRecombinant Human Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3 is produced by our E.coli expression system and the target gene encoding Met1-Ala230 is expressed with a 6His tag at the C-terminus.
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Species reactivityHuman
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OriginEscherichia coli
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Peptide sequenceMEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMDPELLSMVPRPVCAVLLLFPITEKYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGLIHAIANNKDKMHFESGSTLKKFLEESVSMSPEERARYLENYDAIRVTHETSAHEGQTEAPSIDEKVDLHFIALVHVDGHLYELDGRKPFPINHGETSDETLLEDAIEVCKKFMERDPDELRFNAIALSAALEHHHHHH
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Estimated molecular weight27,25 kDa
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Protein purityGreater than 95% as determined by reducing SDS-PAGE.
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Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping conditionDry ice/ice packs
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Package formSupplied as a 0.2 µm filtered solution of 50mM TrisHCl, 150mM NaCl, 1mM DTT, 50% Glycerol, pH 8.0.
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Storage conditionsStore at below -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
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Reconstitution conditionsAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt numberP15374
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolUCHL3, RPL3, LMO7-AS1, KLK9, UCHL1, MRPL38, CCR6, ADARB1
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Short nameRecombinant Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3/UCH-L3 (C-6His)
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameHuman UCH-L3(C-6His)
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameubiquitin C-terminal hydrolase L3
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Synonyms gene name
- ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin C-terminal hydrolases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameribosomal protein L3
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-07-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNA protein coding host genes
- L ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameLMO7 antisense RNA 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2014-03-25
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Entrez gene record
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namekallikrein related peptidase 9
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Synonyms gene name
- kallikrein 9
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-03-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Kallikreins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameubiquitin C-terminal hydrolase L1
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Synonyms gene
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Synonyms gene name
- ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin C-terminal hydrolases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemitochondrial ribosomal protein L38
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-02-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitochondrial ribosomal proteins
- Large subunit mitochondrial ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine receptor 6
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Synonyms gene
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Synonyms gene name
- chemokine (C-C motif) receptor 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-04-21
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Entrez gene record
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Pubmed identfication
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Classification
- C-C motif chemokine receptors
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameadenosine deaminase RNA specific B1
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Synonyms gene name
- adenosine deaminase, RNA-specific, B1 (homolog of rat RED1)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-10-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Adenosine deaminases acting on RNA
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data