anti-DDDDK Antibody [1D1B12] (biotin)

  • Catalog number
    PM-7657-biotin
  • Price
    Please ask
  • Size
    0.1 mg
  • Gene info
    Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. DDDDK-tag is one of the more popular epitope tags for expressed recombinant proteins.
  • Gene names
    Biotin-DDDDK, DDDDK, FLAG, FLAGtag,flag-tag
  • Antibodie s label
    Biotin
  • Purity
    Biotin-DDDDK antibody is affinity chromatography purified via peptide column.
  • Ab raised in
    Mouse
  • Type
    Monoclonal
  • Monoclonal
    1D1B12
  • Immunoglobulin
    IgG
  • Spicific to
    Biotin-DDDDK-tag antibody will detect carboxy-terminal DDDDK-tagged recombinant proteins.
  • Antigen
    Biotin-DDDDK antibody was made against a synthetic peptide containing DYKDDDDK.
  • Immuno application
    ELISA, Western Blot
  • Antibody advice
    Biotin-DDDDK antibody can be used for detection of DDDDK by Western blot at 0.5 - 1 μ/ml.
  • Suspention pH azide
    Biotin-DDDDK antibody is supplied in PBS containing 0.02% sodium azide.
  • Keep at
    Biotin-DDDDK antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year.
  • Works with
    Tag
  • Omim nr
    N/A
  • Ncbi nr
    N/A
  • KDa
    N/A
  • Vial with antibody
    in solution
  • Quantity per ml
    1 mg/mL
  • NCBI GENE nr
    60
  • GENE symbol
    ACTB
  • Antigene name
    actin, beta
  • Source
    homo sapiens
  • Protein nr
    P60709
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • French translation
    anticorps
  • Gene target
    DDDDK   [1D1B12]  
  • Short name
    anti-DDDDK Antibody [1D1B12] ( )
  • Technique
    Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
  • Label
    Biotin
  • Alternative name
    Antibody toDDDDK (antibody to-) [1D1B12] (biotinilated)
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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