anti-DDDDK Antibody [1D1B12] (biotin)
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Catalog number
PM-7657-biotin
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Price
Please ask
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Size
0.1 mg
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Gene info
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. DDDDK-tag is one of the more popular epitope tags for expressed recombinant proteins.
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Gene names
Biotin-DDDDK, DDDDK, FLAG, FLAGtag,flag-tag
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Antibodie s label
Biotin
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Purity
Biotin-DDDDK antibody is affinity chromatography purified via peptide column.
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Ab raised in
Mouse
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Type
Monoclonal
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Monoclonal
1D1B12
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Immunoglobulin
IgG
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Spicific to
Biotin-DDDDK-tag antibody will detect carboxy-terminal DDDDK-tagged recombinant proteins.
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Antigen
Biotin-DDDDK antibody was made against a synthetic peptide containing DYKDDDDK.
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Immuno application
ELISA, Western Blot
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Antibody advice
Biotin-DDDDK antibody can be used for detection of DDDDK by Western blot at 0.5 - 1 μ/ml.
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Suspention pH azide
Biotin-DDDDK antibody is supplied in PBS containing 0.02% sodium azide.
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Keep at
Biotin-DDDDK antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year.
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Works with
Tag
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Omim nr
N/A
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Ncbi nr
N/A
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KDa
N/A
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Vial with antibody
in solution
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Quantity per ml
1 mg/mL
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NCBI GENE nr
60
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GENE symbol
ACTB
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Antigene name
actin, beta
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Source
homo sapiens
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Protein nr
P60709
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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French translation
anticorps
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Gene target
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Short name
anti-DDDDK Antibody [1D1B12] ( )
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Label
Biotin
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Alternative name
Antibody toDDDDK (antibody to-) [1D1B12] (biotinilated)
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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