anti-IgG Antibody [4D2D9G8]
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Catalog number
32-176
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Price
Please ask
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Size
0.1 mg
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Gene info
Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
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Gene names
CD32, FCG2, FCGR2A1, IGFR2, Low affinity immunoglobulin gamma Fc region receptor II-a, CDw32, IgG Fc receptor II-a, FcGR, CD32A, FCGR2
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Antibodie s label
unlabelled serum
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Purity
Antibody is purified by protein A affinity chromatography.
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Ab raised in
Mouse
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Type
Monoclonal
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Monoclonal
4D2D9G8
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Immunoglobulin
IgG1
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Antigen
Chrompure Human IgG, Fc Fragment.
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Immuno application
ELISA, Western Blot
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Antibody advice
Western Blot:1:200 - 1:1,000 ELISA:Propose dilution 1:10,000.Determining optimal working dilutions by titration test.
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Suspention pH azide
Purified antibody in PBS containing 0.03% sodium azide
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Keep at
IgG monoclonal antibody can be stored at -20 grades C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Works with
Human
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Omim nr
160332371
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Ncbi nr
P12318
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NCBI GENE nr
2212
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GENE symbol
FCGR2A
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Antigene name
Fc fragment of IgG, low affinity IIa, receptor (CD32)
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Source
Homo sapiens
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Protein nr
P12318
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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French translation
anticorps
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Gene target
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Short name
anti-IgG Antibody [4D2D9G8]
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Technique
Antibody, anti-, anti, IgG, antibody to, antibodies, antibodies against human proteins, antibodies for, IgGs
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Isotype
IgG, IgG
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Alternative name
Antibody toIgG (antibody to-) [4D2D9G8]
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Alternative technique
antibodies, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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