Drosophila melanogaster Ubiquitin-conjugating enzyme E2-17KDA (UbcD6)

Description

Recombinant Drosophila melanogaster Ubiquitin-conjugating enzyme E2-17KDA(UbcD6) expressed in E.coli
Specifications

Uniprot ID: P25153; Host: Drosophila melanogaster (Fruit fly); Expression system: E.coli; Expression region: 1-151aa; Sequence information: Full Length; Tag: N-terminal 6xHis-SUMO-tagged; Purity: Greater than 90% as determined by SDS-PAGE.
Additional_information

MW: 33.2 kDa; Buffer composition: Tris-based buffer with 50% glycerol.
Storage_and_shipping

Shipped on ice packs (+4°C). The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Repeated freezing and thawing should be avoided. Store working aliquots at 4°C for up to one week.
Notes

For research use only.
Additional description

Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.

Gene target

melanogaster Ubiquitin-conjugating E2-17KDA UbcD6
Gene symbol

DLAT, DBT, SNORA62, UBE2S, DLST, UBE2O, NFE2, CST12P, SDR16C5, CDC34
Short name

melanogaster Ubiquitin-conjugating enzyme E2-17KDA (UbcD6)
Technique

Enzyme, enzymes
Species

Drosophila, Drosophila subspecies d. Megalonaster or the fruit fly is used in genetic research because of the short life cycle and the possibility of knock-out and knock-ins with Cas9 for ends-in gene targets like CRISPR.Cas9 and TALEN vectors . Drosophilas
Alternative name

Drosophila melanogaster Ubiquitin-conjugating enzyme E2-17KDA (UbcD6)
Alternative technique

enzymes

Identity

HGNC:2896
Gene

DLAT
Long gene name

dihydrolipoamide S-acetyltransferase
Synonyms gene
- DLTA
Synonyms
- PDC-E2
- E2
Synonyms name
- E2 component of pyruvate dehydrogenase complex
- dihydrolipoyllysine-residue acetyltransferase
GenBank acession
- Y00978
Locus

11q23.1
Discovery year

1989-06-30
Entrez gene record

1737
Pubmed identfication
- 8102256
RefSeq identity
- NM_001931
Classification
- Pyruvate dehydrogenase complex
VEGA ID

OTTHUMG00000133751

Identity

HGNC:2698
Gene

DBT
Long gene name

dihydrolipoamide branched chain transacylase E2
Synonyms gene name
- dihydrolipoamide branched chain transacylase (E2 component of branched chain keto acid dehydrogenase complex; maple syrup urine disease)
Synonyms
Synonyms name
GenBank acession
- BC016675
Locus

1p21.2
Discovery year

1989-06-30
Entrez gene record

1629
Pubmed identfication
- 1420314
- 1429740
RefSeq identity
- NM_001918
VEGA ID

OTTHUMG00000010921

Identity

HGNC:2911
Gene

DLST
Long gene name

dihydrolipoamide S-succinyltransferase
Synonyms gene
- DLTS
Synonyms gene name
- dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex)
Synonyms
- OGDC-E2
- KGD2
Synonyms name
- Dihydrolipoyllysine-residue succinyltransferase
- 2-oxoglutarate dehydrogenase complex component E2
Locus

14q24.3
Discovery year

1989-06-30
Entrez gene record

1743
Pubmed identfication
- 8009371
VEGA ID

OTTHUMG00000171483

Identity

HGNC:7780
Gene

NFE2
Long gene name

nuclear factor, erythroid 2
Synonyms gene name
- nuclear factor (erythroid-derived 2), 45kD
- nuclear factor (erythroid-derived 2), 45kDa
Synonyms
- NF-E2
GenBank acession
- BC005044
Locus

12q13.13
Discovery year

1993-11-03
Entrez gene record

4778
Pubmed identfication
- 8355703
RefSeq identity
- NM_006163
Classification
- Basic leucine zipper proteins
VEGA ID

OTTHUMG00000169761