Rabbit Anti-Human ATG7 Polyclonal: FITC
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Catalog numberSPC-610D-FITC
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Price:
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Size100 µg
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ImmunogenSynthetic peptide from the N-terminal of Human ATG7
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Antibody s targetHuman ATG7
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Antibody s full descriptionRabbit Anti-Human ATG7 Polyclonal
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Primary research fieldsCancer, Apoptosis, Autophagy, Cardiovascular System, Heart
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameAutophagy 7S. cerevisiae, homolog of Antibody, APG7 like Antibody, ATG7_HUMAN Antibody, hAGP7 Antibody, Ubiquitin-like modifier-activating enzyme ATG7 Antibody, Autophagy related protein 7 Antibody, Autophagy-related protein 7 Antibody, GSA 7 Antibody, APG7, S. cerevisiae, homolog of Antibody, Ubiquitin-activating enzyme E1-like protein Antibody, Autophagy-related 7 (yeast) Antibody, APG7 autophagy 7 like Antibody, 1810013K23Rik Antibody, APG7 autophagy 7-like (S. cerevisiae) Antibody, ATG12-activating enzyme E1 ATG7 Antibody, ATG7 autophagy related 7 homolog Antibody, Atg7l Antibody, Ubiquitin activating enzyme E1 like protein Antibody, Apg 7 Antibody, APG7L Antibody, DKFZp434N0735 Antibody, ATG 7 Antibody, APG7-like Antibody, GSA7 Antibody, ATG7 autophagy related 7 homolog (S. cerevisiae) Antibody, ATG7 Antibody
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Verified applicationsWB
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Raised inRabbit
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s recommended dilutions for useWB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects 77.9 kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-610 was sufficient for detection of ATG7 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm , Preautophagosomal Structure
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Tissue specificityWidely expressed, especially in kidney, liver, lymph nodes and bone marrow.
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Scientific contextATG7 in conjuction with ATG10, mediates the formation of the autophagosome when ATG12 is covalently bound to ATG5 and targets to autophagosome vesicles. It also activates ATG8, and is crucial for amino acid supply in neonates.
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Bibliography1. Mizushima N., et al. (1998) J Biol Chem. 273: 33889-92. 2. Mizushima N., et al. (1998) Nature. 395: 395-8. 3. Suzuki K., et al. (2001) EMBO J. 20: 5971-81. 4. Tanida I., et al. (1999) Mol Biol Cell. 10: 1367-79. 5. Shintani T., et al. (1999) EMBO J. 18: 5234-41.
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Released date3/Jan/2017
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NCBI numberNP_001129503.2
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Gene number10533
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Protein numberO95352
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendWestern blot analysis of Rat brain cell lysates showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody (SPC-610). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain cell lysates. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody (SPC-610) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa. | Western blot analysis of Human Cervical Cancer cell lysates (HeLa) showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody (SPC-610). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical Cancer cell lysates (HeLa). Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody (SPC-610) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa. Rabbit Anti-ATG7 Antibody used in Western blot (WB) on brain cell lysates (SPC-610) | Rabbit Anti-ATG7 Antibody used in Western blot (WB) on Cervical Cancer cell lysates (HeLa) (SPC-610)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesThis StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAnti-FITC Antibody
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolATG7
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Short nameRabbit Anti- ATG7 Polyclonal: FITC
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelFITC
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman ATG7 polyclonal: fluorecein
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Alternative techniquepolyclonals, rabbit-anti, antibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameautophagy related 7
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Synonyms gene
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Synonyms gene name
- APG7 autophagy 7-like (S. cerevisiae)
- ATG7 autophagy related 7 homolog (S. cerevisiae)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-11-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin like modifier activating enzymes
- Autophagy related
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data